This completely revised and updated edition provides a comprehensive overview of mammalian biochemistry. Topics examined include introductions to the structure of the cell and protein composition, followed by in depth coverage of biological membranes, bioenergetics, metabolism of carbohydrates, lipids, amino acids and nucleotides. Chapters have been updated on DNA replication and repair, recombinant DNA and biotechnology, regulation of gene expression and RNA structure and function. Further subjects covered include protein synthesis and post-translational modification, biochemistry of hormones, and biotransformation.