The fourth edition of Gene Cloning is a fully revised and updated version of this most popular introductory text on the subject. This exciting new edition retains the basic structure and organisation that has proved successful for the previous three editions. Two major changes in the contents of the book are the introduction of the subject of the polymerase chain reaction (PCR) at the beginning of the book with more emphasis being placed on PCR throughout and the inclusion of a new chapter on genome sequencing and functional analysis.