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Mass Spectrometry of Proteins and Peptides John Chapman

Mass Spectrometry of Proteins and Peptides von John Chapman

Mass Spectrometry of Proteins and Peptides John Chapman


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Mass Spectrometry of Proteins and Peptides Zusammenfassung

Mass Spectrometry of Proteins and Peptides John Chapman

John R. Chapman recreates the success of his earlier acclaimed book (Protein and Peptide Analysis by Mass Spectrometry, 1996) with a major new collection of cutting-edge methods in the analysis of proteins and peptides. Contributed by well-established investigators, each chapter provides background information before guiding the researcher step-by-step through the experimental process, with detailed instructions to help assure experimental success. The applications covered range widely and include protein sequencing, higher-order structure determination, epitope mapping, kinetics, quantitation, glycosylation analysis, and bacterial typing. Authoritative and eminently practical, Mass Spectrometry of Proteins and Peptides demonstrates the full scope of this versatile analytical technique. All protein chemists and biochemists, as well as bioanalytical scientists, who want to exploit these powerful methods in their work, will find this book indispensable.

Mass Spectrometry of Proteins and Peptides Bewertungen

John Chapman has followed on from his excellent earlier volume Protein and Peptide Analysis by Mass Spectrometry' by bringing together in this both very readable and informative volume 27 individual chapters from acknowledged practitioners of the application of mass spectrometry to peptides and proteins. Each chapter is packed with real information both for the biologist and mass spectroscopist and contains a wealth of detail on sample reparation and the subsequent interpretation of the MS results. The hot' topics of the investigation of non-covalent complexes, protein-protein interactions and higher order protein structures by mass spectrometry are addressed in separate chapters. Any laboratory working on peptides and proteins whether they use MS or not should have access to this book. It is a goldmine. - European Peptide Society Newsletter a well crafted book using the experience of respected pioneers of modern mass spectrometric methods. Many of the methods used in proteomic studies rely on mass spectrometric analysis of proteins and peptides, as well as protein separation technology. The latter is dealt with to some extent by this volume; however, the mass spectrometric aspects are very well covered. This volume shows the great versatility of mass spectrometric analysis, in this case related to protein and peptides, indicating that most analytical challenges can be met by the technique, especially when combined with innovative sample preparation. For the clinical biochemist with an interest in proteins and peptides, this volume is an important reference. For other biochemists it represents a view of a great potential, which will develop as our use of the human genome information progresses. - Annals of Clinical Biochemistry The availability of a compilation of authoritative reviews that include a wealth of practical detail regarding sample preparation and presentation as well as instrument operation is both timely and of considerable value. In total there are some twenty-seven comprehensive descriptions of mass spectrometric analyses of proteins, peptides and mixtures of various complexity and provenance. The elucidation of higher-order structure is described as well as primary sequence determination, epitope mapping and cell typing using several substrates from highly refined isolates to more complex mixtures and whole cells. The contributions are provided by over sixty well established researchers and include detailed information for sample isolation and preparation as well as a comprehensive bibliography on each topic area. Further information is collated into a series of appendices covering an abbreviation glossary, listings of mass/abundance values for elements, amino acids and monosaccharide residues and a literature update on protein mass spectrometry for 1997 - 1998. This volume is a valuable addition to the series: it is comprehensive in terms of the range of applications considered and extensive in the descriptions of the analytical process. It is unusual among reviews of mass spectrometric applications for the wealth of experimental detail provided an this aspect alone makes it an essential item for every biological mass spectrometry and proteomics laboratory. - Chromatographia

Inhaltsverzeichnis

De Novo Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry Using Triple Quadrupole and Quadrupole/Time-of-Flight Instruments Andrej Shevchenko, Igor Chernushevich, Matthias Wilm, and Matthias Mann Direct Analysis of Proteins in Mixtures: Application to Protein Complexes John R. Yates, III, Andrew J. Link, and David Schieltz Characterization of a Mutant Recombinant S100 Protein Using Electrospray Ionization Mass Spectrometry Mark J. Raftery Searching Sequence Databases via De Novo Peptide Sequencing by Tandem Mass Spectrometry Richard S. Johnson and J. Alex Taylor Signature Peptides: From Analytical Chemistry to Functional Genomics Haydar Karaoglu and Ian Humphery-Smith Investigating the Higher Order Structure of Proteins: Hydrogen Exchange, Proteolytic Fragmentation, and Mass Spectrometry John R. Engen and David L. Smith Probing Protein Surface Topology by Chemical Surface Labeling, Crosslinking, and Mass Spectrometry Keiryn L. Bennett, Thomas Matthiesen, and Peter Roepstorff Secondary Structure of Peptide Ions in the Gas Phase Evaluated by MIKE Spectrometry: Relevance to Native Conformations Igor A. Kaltashov, Aiqun Li, Zoltan Szilagyi, Karoly Vekey, and Catherine Fenselau Preparation and Mass Spectrometric Analysis of S-Nitrosohemoglobin Pasquale Ferranti, Gianfranco Mamone, and Antonio Malorni Multiple and Subsequent MALDI-MS On-Target Chemical Reactions for the Characterization of Disulfide Bonds and Primary Structures of Proteins H. Peter Happersberger, Marcus Bantscheff, Stefanie Barbirz, and Michael O. Glocker Epitope Mapping by a Combination of Epitope Excision and MALDI-MS Carol E. Parker and Kenneth B. Tomer Identification of Active Site Residues in Glycosidases by Use of Tandem Mass Spectrometry David J. Vocadlo and Stephen G. Withers Probing Protein Protein Interactions with Mass Spectrometry Richard W. Kriwacki and Gary Siuzdak Studies of Noncovalent Complexes in an Electrospray Ionization/Time-of-Flight Mass Spectrometer Andrew N. Krutchinsky, Ayeda Ayed, Lynda J. Donald, Werner Ens, Harry W. Duckworth, and Kenneth G. Standing Kinetic Analysis of Enzymatic and Nonenzymatic Degradation of Peptides by MALDI-TOFMS Fred Rosche, Jorn Schmidt, Torsten Hoffmann, Robert P. Pauly, Christopher H. S. McIntosh, Raymond A. Pederson, and Hans-Ulrich Demuth Characterization of Protein Glycosylation by MALDI-TOFMS Ekaterina Mirgorodskaya, Thomas N. Krogh, and Peter Roepstorff Positive and Negative Labeling of Human Proinsulin, Insulin and C-Peptide with Stable Isotopes: New Tools for In Vivo Pharmacokinetic and Metabolic Studies Reto Stocklin, Jean-Francois Arrighi, Khan Hoang-Van, Lan Vu, Fabrice Cerini, Nicolas Gilles, Roger Genet, Jan Markusssen, Robin E. Offord, and Keith Rose Identification of Snake Species by Toxin Mass Fingerprinting of Their Venoms Reto Stocklin, Dietrich Mebs, Jean-Claude Boulain, Pierre-Alain Panchaud, Henri Virelizier, and Cecile Gillard-Factor Mass Spectrometric Characterization of the b-Subunit of Human Chorionic Gonadotropin Roderick S. Black and Larry D. Bowers Analysis of Gluten in Foods by MALDI-TOFMS Enrique Mendez, Israel Valdes, and Emilio Camafeita Quantitation of Nucleotidyl Cyclase and Cyclic Nucleotide-Sensitive Protein Kinase Activities by Fast-Atom Bombardment Mass Spectrometry: A Paradigm for Multiple Component Monitoring in Enzyme Incubations by Quantitative Mass Spectrometry Russell P. Newton Influence of Salts, Buffers, Detergents, Solvents, and Matrices on MALDI-MS Protein Analysis in Complex Mixtures K. Olaf Bornsen Sample Preparation Techniques for Peptides and Proteins Analyzed by MALDI-MS Martin Kussmann and Peter Roepstorff Analysis of Hydrophobic Proteins and Peptides by Mass Spectrometry Johann Schaller Analysis of Proteins and Peptides Directly from Biological Fluids by Immunoprecipitation/Mass Spectrometry Sacha N. Uljon, Louis Mazzarelli, Brian T. Chait, and Rong Wang Detection of Molecular Determinants in Complex Biological Systems Using MALDI-TOF Affinity Mass Spectrometry Judy Van de Water and M. Eric Gershwin Rapid Identification of Bacteria Based on Spectral Patterns Using MALDI-TOFMS Jackson O. Lay, Jr. and Ricky D. Holland Appendices John R. Chapman

Zusätzliche Informationen

GOR009984580
9780896036093
089603609X
Mass Spectrometry of Proteins and Peptides John Chapman
Gebraucht - Sehr Gut
Gebundene Ausgabe
Humana Press Inc.
2000-01-01
544
N/A
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