The purpose of this practical book is to provide scientists, whatever their specialty, with an account of the various techniques available for detecting, estimating and isolating biological molecules. The methods presented are based on the range of immune reactions that have been discovered in recent years, the usefulness of which has quickly been put to work in many research programmes, as well as the biotechnological industries. At the forefront of the advances made, has been an improved understanding of the role of monoclonal antibodies. Each chapter in the second edition of Immunochemistry in Practice has been updated to take these advances into consideration. Clear guidance on purifying, digesting and applying fractions has been given along with a wide coverage of immunohistochemistry and immunoblotting. The sections on the immunoradiometric assays, ELISA and IRMA, have also been expanded in this edition.