Part I. Genetics in Vascular Disease Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism Analysis Shu Ye and Adriano M. Henney Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis Ana Cenarro, Fernando Civeira, and Miguel Pocovi Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes Joseph M. Miano, Emilio Garcia, and Ralf Krahe Part II. Isolation of Genes Expressed in Vascular Tissue Efficient Extraction of RNA from Vascular Tissue Catherine F. Townsend, Christopher M. H. Newman, and Sheila E. Francis Preparation of cDNA Libraries from Vascular Cells Mark E. Lieb and Mark B. Taubman Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells Csilla Csortos, Virginie Lazar, and Joe G. N. Garcia Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR Rong-Fong Shen Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques Kerry Tyson and Catherine Shanahan The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells Paul J. Adam Identification of Novel Protein Kinases in Vascular Cells Ian Zachary, Spiros Servos, and Barbara Herren Part III. Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes Yutaka Kitami and Kunio Hiwada Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells Rosalind P. Fabunmi Nuclear Run-On Assayto Study Gene Transcription in Vascular Cells Ulrich Laufs and James K. Liao Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells Todd Bourcier Part IV. Molecular Analysis of mRNA Expression in Vascular Cells Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis Joachim Fruebis Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases Mingyi Chen and Tatsuya Sawamura Localization of Gene Expression in Human Atherosclerotic Lesions by In Situ Hybridization Lee D. K. Buttery and Julia M. Polak RNase Protection Assays for Quantification of Gene Expression in Vascular Tissue Nengyu Yang, Shuli Wang, and James E. Faber Part V. Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissue Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick End Labeling (TUNEL) Mark Kockx, Johannes Muhring, and Michiel Knaapen In Vitro Detection of Apoptosis in Isolated Vascular Cells Shiu-Wan Chan and Martin R. Bennett Molecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence Analysis Rachel Johnatty, Giulio Gabbiani, and Pascal Neuville Part VI. In Vitro Methods to Express Foreign Genes in Vascular Cells Simple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular Cells Stuart A. Nicklin and Andrew H. Baker Generation of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular Cells Carmel M. Lynch Hemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular Cells Yoshikazu Yonemitsu and Yasufumi Kaneda High-Efficien
