Part I: Human Immunodeficiency Virus Type 1 (HIV-1)
1. A Fluorescence-Based High Throughput Screening Assay to Identify HIV-1 Inhibitors
Peggy Geluykens, Koen Van Acker, Johan Vingerhoets, Christel Van den Eynde, Marnix Van Loock, and Gery Dams
2. A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion
Liesbet Smeulders, Lieve Bunkens, Inge Vereycken, Koen Van Acker, Pascale Holemans, Emmanuel Gustin, Marnix Van Loock, and Gery Dams
3. Identification of HIV-1 Reverse Transcriptase Inhibitors Using a Scintillation Proximity Assay
Bertrand Van Schoubroeck, Marnix Van Loock, Tania Ivens, Pascale Dehertogh, Dirk Jochmans, and Gery Dams
4. Biochemical Screening Assays to Identify HIV-1 Integrase Inhibitors
Marleen Clynhens, Alexandra Smets, Inge Vereycken, Marnix Van Loock, Reginald Clayton, Geert Meersseman, and Olivia Goethals
5. HIV-1 Genotyping of the Protease-Reverse Transcriptase and Integrase Genes to Detect Mutations that Confer Anti-Retroviral Resistance
Peter Van den Eede, Liesbeth Van Wesenbeeck, Yvan Verlinden, Maxim Feyaerts, Veerle Smits, Ann Verheyen, Leen Vanhooren, Alain Deloof, Jorge Villacian, and Theresa Pattery
Part II: Hepatitis Viruses
6. In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis
Rumin Zhang and William T. Windsor
7. A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template
Eric Ferrari and Hsueh-Cheng Huang
8. Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon
Inge Vliegen, Leen Delang, and Johan Neyts
9. A Cellular Replicon-Based Phenotyping Assay to Determine Susceptibility of Hepatitis C Virus Clinical Isolates to NS3/4A Protease Inhibitors
Leen Vijgen, Jannick Verbeeck, Barbara Van Kerckhove, Jan Martin Berke, Diana Koletzki, Gregory Fanning, and Oliver Lenz
10. Expression and Purification of Hepatitis C Virus Protease from Clinical Samples
Liesbet van der Helm
11. Surface Plasmon Resonance as a Tool to Select Potent Drug Candidates for Hepatitis C Virus NS5B Polymerase
Erna Cleiren, Benoit Devogelaere, and Katleen Fierens
12. Amplification and Sequencing of the Hepatitis C Virus NS3/4A Protease and the NS5B Polymerase Regions for Genotypic Resistance Detection of Clinical Isolates of Subtypes 1a and 1b
Diana Koletzki, Theresa Pattery, Bart Fevery, Leen Vanhooren, and Lieven J. Stuyver
13. A Southern Blot Assay for Detection of Hepatitis B Virus Covalently Closed Circular DNA from Cell Cultures
Dawei Cai, Hui Nie, Ran Yan, Ju-Tao Guo, Timothy M. Block, and Haitao Guo
14. In Vitro Phenotyping of Recombinant Hepatitis B Virus Containing the Polymerase/Reverse Transcriptase Gene from Clinical Isolates
Yang Liu and Kathryn M. Kitrinos
Part III: Flaviviruses
15. Cell-Based Antiviral Assays for Screening and Profiling Inhibitors Against Dengue Virus
Edwin Yunhao Gong, Marleen Clynhens, Tania Ivens, Pedro Lory, Kenny Simmen, and Guenter Kraus
16. A Duplex Real-Time RT-PCR Assay for Profiling Inhibitors of Four Dengue Serotypes
Edwin Yunhao Gong, Alexandra Smets, Nick Verheyen, Marleen Clynhens, Emmanuel Gustin, Pedro Lory, and Guenter Kraus
17. High Throughput Screening Using Dengue Virus Reporter Genomes
Wolfgang Fischl and Ralf Bartenschlager
18. Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using a FRET Substrate
Christoph Nitsche and Christian D. Klein
19. Expression and Purification of Dengue Virus NS5 Polymerase and Development of a High Throughput Enzymatic Assay for Screening Inhibitors of Dengue Polymerase
Edwin Yunhao Gong, Hannah Kenens, Tania Ivens, Koen Dockx, Katrien Vermeiren, Genevieve Vandercruyssen, Benoit Devogelaere, Pedro Lory, and Guenter Kraus
20. Detection and Quantification of Flavivirus NS5 Methyl-transferase Activities
Siew Pheng Lim, Christophe Bodenreider , and Pei-Yong Shi
21. Testing Antiviral Compounds in a Dengue Mouse Model
Wouter Schul, Andy Yip, and Pei-Yong Shi
22. Construction of Self-Replicating Subgenomic West Nile Virus Replicons for Screening Antiviral Compounds
Sofia L. Alcaraz-Estrada, Erin Donohue Reichert, and Radhakrishnan Padmanabhan
Part IV: Herpes Viruses
23. Methods for Screening and Profiling Inhibitors of Herpes Simplex Viruses
Edwin Yunhao Gong
24. In Vivo Evaluation of Antiviral Efficacy Against Genital Herpes Using Mouse and Guinea Pig Models
Frances Valencia, Ronald L. Veselenak, and Nigel Bourne
25. A Fluorescence-Based High Throughput Screening Assay for Identifying Human Cytomegalovirus Inhibitors
Christel Van den Eynde, Ellen Van Damme, Tania Ivens, and Edwin Yunhao Gong
Part V: Respiratory Viruses
26. A Fluorescence-Based High Throughput Antiviral Compound Screening Assay Against Respiratory Syncytial Virus
Leen Kwanten, Ben De Clerck, and Dirk Roymans
27. Screening and Evaluation of Anti-Respiratory Syncytial Virus Compounds in Cultured Cells
Anna Lundin, Tomas Bergstroem, and Edward Trybala
28. Evaluation of Antiviral Efficacy Against Human Respiratory Syncytial Virus Using Cotton Rat and Mouse Models
Joke Van den Berg, Leen Kwanten, and Dirk Roymans
29. In Vivo Evaluation of Antiviral Compounds on Respiratory Syncytial Virus Using a Juvenile Vervet Monkey (Chlorocebus pygerythrus) Infection Model
Lieselot Houspie, Hans Stevens, Maina Ngotho, Els Keyaerts, Gabriela Ispas, Rene Verloes, Marc Van Ranst, and Piet Maes
30. Methods to Determine Mechanism of Action of Anti-Influenza Inhibitors
Angela Luttick, Stephanie Hamilton, and Simon P. Tucker
31. Methods for Evaluation of Antiviral Efficacy Against Influenza Virus Infections in Animal Models
Donald F. Smee and Dale L. Barnard
Part VI: Alphaviruses
32. Development of a High Throughput Antiviral Assay for Screening Inhibitors of Chikungunya Virus and Generation of Drug-Resistant Mutations in Cultured Cells
Edwin Yunhao Gong, Jean-Francois Bonfanti, Tania Ivens, Marijke Van der Auwera, Barbara Van Kerckhove, and Guenter Kraus
33. A Mouse Model of Chikungunya Virus with Utility in Antiviral Studies
Ashley Dagley and Justin G. Julander
