1. The Major Histocompatibility Complex - A Paradigm for Studies of the Human Genome
Richard J. N. Allcock
2. HLA Typing by SSO and SSP Methods
Heather Dunckley
3. Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity
Michael D. Varney, Alison S. L. Castley, Katri Haimila and Paivi Saavalainen
4. HLA Typing Using Bead Based Methods
Daniel Trajanoski and Samantha J. Fidler
5. HLA Typing by Direct DNA Sequencing
Linda K. Smith
6. Data Analysis of HLA Sequencing using Assign-SBT v3.6+ from Conexio
Carla Wirtz and David Sayer
7. Simple Methods for the Detection of HLA-G Variants in Coding and Non-Coding Regions
Holger Nuckel, Erick C. Castelli, Philippe Moreau, Crista Ochsenfarth, Peter A. Horn, and Vera Rebmann
8. Molecular Typing of HLA-E
Nina Lauterbach, Christina E.M. Voorter, and Marcel G.J. Tilanus
9. Molecular Analysis of Complement Component C4 Gene Copy Number
Alison Castley and Olga Patricia Martinez
10. Genotyping of Single Nucleotide Polymorphisms (SNPs) by 5' Nuclease Allelic Discrimination
Mari Malkki and Effie W. Petersdorf
11. High Resolution MICA Genotyping by Sequence-Based Typing (SBT)
Yizhou Zou and Peter Stastny
12. Standard Methods for the Management of Immunogenetic Data
Pierre-Antoine Gourraud, Jill A. Hollenbach, Thomas Barnetche, Richard M. Single, and Steven J. Mack
13. Analytical Methods for Immunogenetic Population Data
Steven J. Mack, Pierre-Antoine Gourraud, Richard M. Single, Glenys Thomson, and Jill A. Hollenbach
14. Analytical Methods for Disease Association Studies with Immunogenetic Data
Jill A. Hollenbach, Steven J. Mack, Glenys Thomson, and Pierre-Antoine Gourraud
15. Impact of HLA Matching and HLA Antibodies in Organ Transplantation: a Collaborative Transplant Study View
Caner Susal and Gerhard Opelz
16. Screening for Antibodies Against MICA by Luminex Flow Cytometry
Yizhou Zou and Peter Stastny
17. HLA Antibody Detection and Characterization by Solid Phase Immunoassays - Methods and Pitfalls
Andrea A. Zachary, Renato M. Vega, Donna P. Lucas, and Mary S. Leffell
18. Detection and Characterisation of Alloreactive T Cells
Mandvi Bharadwaj, Nicole A. Mifsud, and James McCluskey
19. Detection of Allo-HLA Crossreactivity by Viral Specific Memory T-Cell Clones using Single HLA Transfected K562 Cells
Lloyd J. D'Orsogna, Ellen M.W. van der Meer-Prins, Yvonne M. Zoet, Dave L. Roelen, Ilias I.N. Doxiadis, and Frans H.J. Claas
20. Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node
Gabriella Tassone and Samantha J. Fidler
21. Crossmatching by Complement-Dependent Lymphocytotoxicity (CDC)
Samantha J. Fidler
22. The Lymphocyte Crossmatch by Flow Cytometry for Kidney Transplantation
Jonathan Downing
23. Overview of the Killer Cell Immunoglobulin-Like Receptor (KIR) System
Raja Rajalingam
24. KIR Typing by Non Sequencing Methods: Polymerase-Chain Reaction with Sequence-Specific Primers (PCR-SSP)
David Ordonez, Manuela Moraru, Natalia Gomez-Lozano, Elisa Cisneros, and Carlos Vilches
25. Killer Cell Immunoglobulin-Like Receptors (KIR) Typing by DNA Sequencing
Lihua Hou, Minghua Chen, Noriko Steiner, Kanthi Kariyawasam, Jennifer Ng, and Carolyn K. Hurley
26. An Overview of Methods Required to Evaluate Donor NK Cell Alloreactivity for Haploidentical Haemopoietic Stem Cell Transplantation
Andrea Velardi
27. The Detection of NK Cell Alloreactivity by Flow Cytometric CD107a Assay
Dianne De Santis, Bree Foley, Campbell S. Wit, and Frank T. Christiansen
28. Clinical Production and Therapeutic Applications of Alloreactive Natural Killer Cells
David H. McKenna, Diane M. Kadidlo, Sarah Cooley, and Jeffrey S. Miller
29. Minor Histocompatibility Antigen Typing by DNA Sequencing for Clinical Practice in Hematopoietic Stem-Cell Transplantation
Eric Spierings and Els Goulmy
30. Donor Registries and Search Strategies
Carolyn K. Hurley, Machteld Oudshoorn, and Michelle Setterholm
31. Cytokine Gene Polymorphisms: Methods of Detection and Biological Significance
Gurvinder Kaur and Narinder Mehra
32. IMGT (R) Tools for the Nucleotide Analysis of Immunoglobulin (IG) and T Cell Receptor (TR) V-(D)-J Repertoires, Polymorphisms, and IG Mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS
Eltaf Alamyar, Patrice Duroux, Marie-Paule Lefranc, and Veronique Giudicelli
33. IMGT/DomainGapAlign: the IMGT (R) Tool for the Analysis of IG, TR, MH, IgSF and MhSF Domain Amino Acid Polymorphism
Francois Ehrenman and Marie-Paule Lefranc
34. Human Gm, Km and Am Allotypes and their Molecular Characterization: a Remarkable Demonstration of Polymorphism
Marie-Paule Lefranc and Gerard Lefranc