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Plant Genotyping Jacqueline Batley

Plant Genotyping By Jacqueline Batley

Plant Genotyping by Jacqueline Batley


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Summary

The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers.

Plant Genotyping Summary

Plant Genotyping: Methods and Protocols by Jacqueline Batley

The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Plant Genotyping: Methods and Protocols is aimed at plant molecular biologists, geneticists, plant breeders and ecologists who have a target question and need to know the most suitable markers and genotyping system to use.

Table of Contents

1. Advances in Plant Genotyping - Where the Future Will Take Us

Dhwani A. Patel, Manuel Zander, Jessica Dalton-Morgan, and Jacqueline Batley

2. Molecular Marker Applications in Plants

Alice C. Hayward, Reece Tollenaere, Jessica Dalton-Morgan, and Jacqueline Batley

3. Bioinformatics: Identification of Markers from Next Generation Sequence Data

Pradeep Ruperao and David Edwards

4. Molecular Marker Databases

Kaitao Lai, Michal Lorenc, and David Edwards

5. Plant Genotyping Using Fluorescently Tagged Inter Simple Sequence Repeats (ISSRs): Basic Principles and Methodology

Linda M. Prince

6. SSR Genotyping

Annaliese S. Mason

7. Genotyping Analysis Using an RFLP Assay

Shutao Dai and Yan Long

8. DNA Barcoding for Plants

&

nbsp; Natasha de Vere, Tim C.G. Rich, Sarah A. Trinder and Charlotte Long

9. Multiplexed Digital Gene Expression Analysis for Genetical Genomics in Large Plant Populations

Christian Obermeier, Bertha M. Salazar-Colqui, Viola Spamer, and Rod Snowdon

10. SNP Genotyping by Heteroduplex Analysis

Norma Paniego, Corina Fusari, Veronica Lia, and Andrea Puebla

11. Application of the High-Resolution Melting Technique for Gene Mapping and SNP Detection in Plants

David Chagne

12. Challenges of Genotyping Polyploid Species

Annaliese S. Mason

13. Genomic Reduction Assisted Single Nucleotide Polymorphism Discovery using 454-Pyrosequencing

Peter J. Maughan, Joshua A. Udall, and Eric N. Jellen

14. Inter-SINE Amplified Polymorphism (ISAP) for Rapid and Robust Plant Genotyping

Torsten Wenke, Kathrin M. Seibt, Thomas Doebel, Katja Muders, and Thomas Schmidt

15. Screening of Mutations by TILLING in Plants

Nian Wang and Lei Shi

16. Gene Analysis Using Mass Spectrometric Cleaved Amplified Polymorphic Sequence (MS-CAPS) with Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectro

metry (MALSI-TOF)

Hideyuki Kajiwara

17. Quantitative SNP Genotyping of Polyploids with MassARRAY and Other Platforms

Marcelo Mollinari and Oliver Serang

18. SNP Genotyping using KASPar Assays

Scott M. Smith and Peter J. Maughan

19. Skim Based Genotyping by Sequencing

Agnieszka A. Golicz, Philipp E. Bayer, and David Edwards

20. The Restriction Enzyme Target Approach to Genotyping by Sequencing (GBS)

Elena Hilario

21. Methods for the Design, Implementation and Analysis of Illumina Infinium (TM) SNP Assays in Plants

David Chagne, Luca Bianco, Cindy Lawley, Diego Micheletti, and Jeanne M.E. Jacobs

22. Use of the Illumina GoldenGate Assay for Single Nucleotide Polymorphism (SNP) Genotyping in Cereal Crops

Shiaoman Chao and Cindy Lawley

Additional information

NLS9781493943678
9781493943678
1493943677
Plant Genotyping: Methods and Protocols by Jacqueline Batley
New
Paperback
Humana Press Inc.
2016-08-23
315
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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