Part I: Introduction
1. Zymography Principles
Jeff Wilkesman and Liliana Kurz
Part II: Endopeptidase Zymography
2. Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography
Hidetaro Yasumitsu
3. Cysteine Protease Zymography: Brief Review
Jeff Wilkesman
4. Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography
Gavin Kernaghan and Michael Mayerhofer
5. Detection of Aspartic Proteinase Activities Using Gel Zymography
Handunge Kumudu Irani Perera
6. MMP Activity Detection in Zymograms Peter Bencsik, Monika Bartekova, Aniko Goerbe, Krisztina Kiss, Janos Paloczi, Jana Radosinska, Gergo Szucs, and Peter Ferdinandy
7. Characterization of Novel Collagenolytic Proteases
Goran Mucic, Brankica Raskovic, and Natalija Polovic
8. Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors
Zongli Ren, Juanjuan Chen, and Raouf A. Khalil
9. Detection and Characterization of Bacterial Proteinases Using Zymography
Madathiparambil G. Madanan and Ambili Mechoor
10. A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography
Shivcharan Prasad and Ipsita Roy
Part III: Reverse Zymography and In Situ Zymography
11. Reverse Zymography: Overview and Pitfalls
Kanika Sharma and Debasish Bhattacharyya
12. Cell In Situ Zymography: Imaging Enzyme-Substrate Interactions
Aastha Chhabra and Vibha Rani
Part IV: 2D Zymography
13. Examination of Gelatinase Isoforms in Rodent Models of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography
Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui, and Zezong Gu
14. Two-Dimensional Zymography of Proteases from Steatotic Duck Liver
Jeff Wilkesman, Maria Fernanda Padron, Liliana Kurz, and Herve Remignon
Part V: Special Zymography Cases
15. Simultaneous Detection of Activity and Relative Molecular Mass of Adenylate Kinases after SDS-PAGE and Blotting
Silvia Ravera and Isabella Panfoli
16. Silver-Stained Fibrin Zymography: Separation of Proteases and Activity Detection Using a Single Substrate-Containing Gel
Chang-Su Park, Dae-Ook Kang, and Nack-Shick Choi
17. Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for Yeast Species Identification
Esther Gamero-Sandemetrio, Rocio Gomez-Pastor, and Emilia Matallana
18. Detection of Guaiacol Peroxidase on Electrophoretic Gels
Diana Castro, Lellys M. Contreras, Liliana Kurz, and Jeff Wilkesman
19. In Situ Demonstration and Characteristic Analysis of the Protease Using Substrate Immersing Zymography
He HaiLun, Li Hao, and Liu Dan
20. Use of Zymography in Trypanosomiasis Studies
Jessyka Fernanda Santiago Monte, Claudia Jassica Goncalves Moreno, Joana Patricia Molato Figueiredo Lopes Monteiro, Hugo Alexandre de Oliveira Rocha, Aline Rimoldi Ribeiro, and Marcelo Sousa Silva
21. Zymography in Multiwells for Quality Assessment of Proteinases
Ambili Mechoor and Madanan G. Madathiparambil
22. Visualization of Enzyme Activities in Earthworm Biopores by In Situ Soil Zymography
Bahar S. Razavi, Duyen Hoang, and Yakov Kuzyakov
23. Multiplex Cathepsin Zymography to Detect Amounts of Active Cathepsins K, L, S, and V
Manu O. Platt
24. Transfer Zymography
Daniel Pan, Karl A. Wilson, and Anna Tan-Wilson
25. Sequential Detection of Thermophilic Lipase and Protease by Zymography
Liliana Kurz, Zully Hernandez, Lellys M. Contreras, and Jeff Wilkesman
26. Calpain Zymography: General Methodology and Protocol
Kevin K.W. Wang
27. CTAB Zymography for the Analysis of Aspartic Proteases from Marine Sponges
Oscar Gonzalez and Jeff Wilkesman
28. Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase/Lipase Activity
Maria Tapizquent, Marleny Fernandez, Georgina Barreto, Zully Hernandez, L. Mariela Contreras, Liliana Kurz, and Jeff Wilkesman
29. Amylase Zymography
Adarelys J. Andrades Mundo and Lellys M. Contreras