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Diagnostic Bacteriology Kimberly A. Bishop-Lilly

Diagnostic Bacteriology By Kimberly A. Bishop-Lilly

Diagnostic Bacteriology by Kimberly A. Bishop-Lilly


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Summary

This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike.

Diagnostic Bacteriology Summary

Diagnostic Bacteriology: Methods and Protocols by Kimberly A. Bishop-Lilly

This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be applied to diverse or even unknown pathogens, digital PCR, next generation sequencing, and bioinformatic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Diagnostic Bacteriology: Methods and Protocols delivers a wide range of assay types all on the cutting edge of diagnostic bacteriology.

Table of Contents

1. Whole Genome Enrichment using RNA Probes and Sequencing of Chlamydia Trachomatis Directly from Clinical Samples

Amanda Claire Brown and Mette T. Christiansen

2. Characterization of Sinus Microbiota by 16S Sequencing from Swabs

Thad W. Vickery, Jennifer M. Kofonow, and Vijay R. Ramakrishnan

3. Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR)

Veronique Wuyts, Wesley Mattheus, Nancy H.C. Roosens, Kathleen Marchal, Sophie Bertrand, and Sigrid C.J. De Keersmaecker

4. Detection of Helicobacter pylori DNA in Formalin-fixed Paraffin Embedded Gastric Biopsies using Laser Micro-dissection and qPCR

Maria Fernanda Loayza Villa, Valeria Liliana Herrera Sevilla, and Nicolas Vivar Diaz

5. Mycobacterial Load Assay

Stephen H. Gillespie, Wilber Sabiiti, and Katarina Oravcova

6. Defining Diagnostic Biomarkers using Shotgun Proteomics and MALDI-TOF Mass Spectrometry

Jean Armengaud

7. Detection and Typing of 'Candidatus Phytoplasma' spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres

Edel Perez-Lopez, Christine Hammond, Chrystel Olivier, and Tim J. Dumonceaux

8. Detection of Helicobacter Pylori in the Gastric Mucosa by Fluorescence in vivo Hybridization

Silvia Fontenete, Marina Leite, Ceu Figueiredo, Paul Cos, and Nuno F. Azevedo

9. Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections

Anja Mezger, Mats Nilsson, and Dan I. Andersson

10. Detection and Differentiation of Lyme Spirochetes and other Tick-borne Pathogens from Blood using real-time PCR with Molecular Beacons

Samantha Schlachter, Kamfai Chan, Salvatore A. E. Marras, and Nikhat Parveen

11. Methods for Real-time PCR-based Diagnosis of Chlamydia Pneumoniae, Chlamydia Psittaci and Chlamydia Abortus Infections in an Opened Molecular Diagnostic Platform

Onya Opota, Rene Brouillet, Gilbert Greub, and Katia Jaton

12. Real-time PCR to Identify Staphylococci and Assay for Virulence from Blood

Charles E. Okolie

13. Multiplex Peptide Nucleic Acid Fluorescence in situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis

Antonio Machado and Nuno Cerca

14. A Closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium Avium Subsp. Paratuberculosis

Marcos D. Trangoni, Andrea K. Gioffre, and Silvio L. Cravero

15. Highly Specific Ligation-Dependent Microarray Detection of Single Nucleotide Polymorphisms

Noa Wolff and Ivan Barisic

16. Multilocus Sequence Typing (MLST) for Cronobacter spp.

17. Diagnostic Bacteriology: Raman Spectroscopy

Rebecca L. Pavlicek, Nicole J. Crane, Meron Ghebremedhin, Katherine Cilwa, and Eric A. Elster

Additional information

NLS9781493983759
9781493983759
149398375X
Diagnostic Bacteriology: Methods and Protocols by Kimberly A. Bishop-Lilly
New
Paperback
Humana Press Inc.
2018-08-12
264
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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