Name: CRISPR-Cas Methods By M. Tofazzal Islam
SKU: 9781071616598
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CRISPR-Cas Methods Summary

CRISPR-Cas Methods: Volume 2 by M. Tofazzal Islam

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis.

Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism.

The chapter CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

A Complete Methodology for the Instruction of CRISPR-based Gene Editing Using a Simplified Cell-free Extract System with Genetic Readout in Bacteria.-In silico Analysis of gRNA Secondary Structure to Predict Its Efficacyfor Plant Genome Editing.-Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants.-CRISPR-dCas9 Based Targeted Manipulation of DNA Methylation in Plants.-Rapid Assembly of Multiplex Natural CRISPR Arrays.-Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants.-Designing, Performing and Analysing CRISPR-Cas9-mediated Genome Editing Experiments in Leguminous Plant.-Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9.-Targeted Base Editing in Soybean using a CRISPR-Cas9 Cytidine Deaminase Fusion.-Genome Editing in Potato Using a Hairy Root Transformation System.-Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato.-CRISPR-Cas9-Mediated Genome Editing in Rice: A Systematic Protocol for Single and Multi-Target Vector Construction.-Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9.-CRISPR-Cas12a-based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants.-CRISPR-Cas9-mediated Gene Editing in Human Induced Pluripotent Stem Cells.-Cell-Type-Specific CRISPR-Cas9 System with miRNAs.-CRISPR-Cas9 gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-puro Vectors.-An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA that Regulate CRISPR-Cas systems.-Genetic Engineering of a Phage-based Delivery System for Endogenous III-A CRISPR-Cas System against Mycobacterium tuberculosis.-CRISPR-Cas9-Mediated Genome Editing in Escherichia coli Bacteriophages.-CRISPR-Cas Genome Editing in the Cellulolytic Bacterium Clostridium thermocellum.-Gene Targeting in Caenorhabditis elegans Using a Combination of Multiple sgRNAs and a Homologous Recombination-mediated Repair.-Design of Repair Templates for CRISPR-Cas9-triggered Homologous Recombination in Caenorhabditis elegans.

Additional information

Sku

NPB9781071616598

ISBN 13

9781071616598

ISBN 10

1071616595

Title

CRISPR-Cas Methods: Volume 2 by M. Tofazzal Islam

Author

M. Tofazzal Islam

Series

Springer Protocols Handbooks

Condition

New

Binding type

Paperback

Publisher

Springer-Verlag New York Inc.

Year published

2022-07-31

Number of pages

397

Prizes

N/A

Cover note

Book picture is for illustrative purposes only, actual binding, cover or edition may vary.

Note

This is a new book - be the first to read this copy. With untouched pages and a perfect binding, your brand new copy is ready to be opened for the first time

CRISPR-Cas Methods M. Tofazzal Islam

CRISPR-Cas Methods by M. Tofazzal Islam

Summary

CRISPR-Cas Methods Summary

CRISPR-Cas Methods: Volume 2 by M. Tofazzal Islam

Table of Contents

Additional information

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