Biopolymer-Based Nano Films: Applications in Food Packaging and Wound Healing covers a variety of biofilms, including active biofilms, nisin-silver nano-films, silk fibroin-based composite films, lignocellulose/cellulose-based biofilms, carboxymethyl cellulose-coated polypropylene, hybrid film-loaded antimicrobials, chitosan hybrid systems, pullulan, and biopolymers films. The applications of these nano-biofilms in different fields, particularly in food packaging, wound healing, and as potential antimicrobials against new, emerging, and multidrug resistant microbes are also discussed. This is an important resource for researchers in the fields of pharmacology, nanotechnology, microbiology, biotechnology, and for clinicians. The possibility of associating nanotechnology with biotechnology helps with the creation of innovative new products and the development of processes at the molecular level. Within this context, nanobiotechnology advances and revolutionizes several scientific fields. In the development of new technologies and products, it is also necessary to develop platforms that allow the specific application and delivery of compounds/actives in a controlled, specific and non-toxic way.