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Immunochemistry in Practice Prof Alan P Johnstone (George Mason University, Virginia USA)

Immunochemistry in Practice By Prof Alan P Johnstone (George Mason University, Virginia USA)

Immunochemistry in Practice by Prof Alan P Johnstone (George Mason University, Virginia USA)


$24.99
Condition - Very Good
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Summary

This work provides scientists of all disciplines with clear descriptions of the wide range of immunochemical techniques available for detecting, isolating and characterizing biological molecules. It explains how to produce and purify antibodies, and how to tag them for later visualization.

Immunochemistry in Practice Summary

Immunochemistry in Practice by Prof Alan P Johnstone (George Mason University, Virginia USA)

This practical book provides scientists of all disciplines with clear descriptions of the plethora of immunochemical techniques available for detecting, isolating and characterising biological molecules. The revolution in molecular biology and biotechnology in the past decade has meant that immunochemistry has become an integral part of the experimental armoury of scientists from a variety of backgrounds. At the heart of this technology is the remarkable specificity that antibodies display toward their epitopes. The specific recognition offered by immunological molecules has resulted in their uptake for a wide range of purposes. This book explains how to produce and purify antibodies and how to tag them for later visualisation.

Table of Contents

1. Basic techniques; 2. Production of antibodies; 3. Purification of immunoglobulins, constituent chains and fragments; 4. Isolation and fractionation of lymphocytes; 5. Radiolabelling techniques; 6. Precipitation techniques in agar and agarose; 7. Analytical electrophoresis and isoelectric focusing; 8. Specific detection of antigens separated by polyacrylamide gel electrophoresis; 9. Preparative gel techniques; 10. Affinity chromatography and immunoprecipitation; 11. Coupling antibodies to fluorochromes, enzymes and biotin; 12. Immunoassays; 13. Immunocytochemistry, immunohistochemistry and flow cytofluorimetry; Appendix 1: Abbreviations; Appendix 2: Buffers; Appendix 3: Manufacturers and suppliers; References; Index

Additional information

GOR006117712
9780865426337
0865426333
Immunochemistry in Practice by Prof Alan P Johnstone (George Mason University, Virginia USA)
Used - Very Good
Paperback
John Wiley and Sons Ltd
1996-04-18
384
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
This is a used book - there is no escaping the fact it has been read by someone else and it will show signs of wear and previous use. Overall we expect it to be in very good condition, but if you are not entirely satisfied please get in touch with us

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