Preface...
Table of Contents...
Contributors...
Part I Wheat Rust Surveillance and Genotyping
1. Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping
Sajid Ali and David Hodson
2. Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance Vanessa Bueno-Sancho, Daniel C.E. Bunting, Luis J. Yanes, Kentaro Yoshida, and Diane G.O. Saunders
3. Race Typing of Puccinia striiformis
on Wheat Mogens S. Hovmoller, Julian Rodriguez-Algaba, Tine Thach, and Chris K. Sorensen
4. Assessment of Aggressiveness of Puccinina striiformis on Wheat Chris K. Sorensen, Tine Thach, and Mogens S. Hovmoller
5. Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing Benjamin Schwessinger and John P. Rathjen
6. Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis
Sajid Ali, Muhammad R. Khan, Angelique Gautier, Zahoor A. Swati, and Stephanie Walter
Part II Molecular Pathogenicity
7. Computational Methods for Predicting Effectors in Rust Pathogens
Jana Sperschneider, Peter N, Dodds, Jennifer M. Taylor, and Sebastien Duplessis
8. Protein-Protein Interaction Assays with Effector-GFP Fusions in Nicotiana benthamiana
Benjamin Petre, Joe Win, Frank L.H. Menke, and Sophien Kamoun
9. Proteome Profiling by 2D-Liquid Chromatography for Wheat-Rust Interaction
Semra Hasancebi
10. Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing
Vinay Panwar and Guus Bakkeren
11. Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi
Veronica Roman-Reyna and John P. Rathjen
Part III Genetics of Rust Resistance
12. Genetic Analysis of Resistance to Wheat Rusts
Caixia Lan, Mandeep Singh Randhawa, Julio Huerta-Espino, and Ravi P. Singh
13. Advances in Identification and Mapping of Rust Resistance Genes in Wheat
Urmil Bansal and Harbans Bariana
14. Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives
Peng Zhang, Ian S. Dundas, Steven S. Xu, Bernd Friebe, Robert A. McIntosh, and W.J. Raupp
15. Application of Genomic Selection in Breeding Wheat for Rust Resistance
Leonardo Ornella, Juan Manuel Gonzalez-Camacho, Susanne Dreisigacker, and Jose Crossa
16. Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown Under Controlled Conditions
Adnan Riaz and Lee Hickey
Part IV Rust Resistance Gene Isolation and Characterization
17. Generation of Loss of Function Mutants for Wheat Rust Disease Resistance Gene Cloning
Rohit Mago, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B.H. Wulff, and Evans Lagudah
18. Isolation of Wheat Genomic DNA for Gene Mapping and Cloning
Guotai Yu, Asyraf Hatta, Sambasivam Periyannan, Evans Lagudah, and Brande B.H. Wulff
19. MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
Burkhard Steuernagel, Kamil Witek, Jonathan D.G. Jones, and Brande B.H. Wulff
20. Rapid Gene Isolation using MutChromSeq
Burkhard Steuernagel, Jan Vrana, Miroslava Karafiatova, Brande B.H. Wulff, and Jaroslav Dolezel
21. Rapid Identification of Rust Resistance Genes through Cultivar-Specific de novo Chromosome Assemblies
Anupriya K. Thind, Thomas Wicker, and Simon G. Krattinger
22. BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance
Li Huang
23. Yeast as a Heterologous System to Functionally Characterize a Multiple Rust Resistance Gene which Encodes a Hexose Transporter
Ricky J. Milne, Katherine E. Dibley, and Evans S. Lagudah
Part V Biocontrol Agents for Rust Management
24. Biocontrol Agents for Controlling Wheat Rust
Siliang Huang and Fahu Pang