Part I: Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology
1. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun
Caixia Gao and Klaus K. Nielsen
2. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery
Shoko Ueki, Shimpei Magori, Benoit Lacroix, and Vitaly Citovsky
3. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues
Mulpuri Sujatha and K.B.R.S. Visarada
4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun)
Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen
5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies
Victor Gaba, Moshe Lapidot, and Amit Gal-On
6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun
Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn
Part II: Biolistic DNA Delivery in Nematodes
7. Biolistic Transformation of Caenorhabditis elegans
Meltem Isik and Eugene Berezikov
8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans
Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher
9. Biolistic Transformation of Brugia malayi
Tarig B. Higazi and Thomas R. Unnasch
Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro
10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells
Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens
11. Biolistic Transfection of Tumor Tissue Samples
Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang
12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes
David F. Steele, Ying Dou, and David Fedida
13. Biolistic Transfection of Neurons in Organotypic Brain Slices
John A. O'Brien and Sarah C.R. Lummis
Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun
Meng-Chi Yen and Ming-Derg Lai
15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery
Ahmad Jalili
16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs
Steve Pascolo
17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter
Yvonne Hoehn, Stephan Sudowe, and Angelika B. Reske-Kunz
18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice
Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus
Part V: Biolistic DNA Vaccination in Animal Disease Models
19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets
Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller
20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models
Gustaf Ahlen, Matti Sallberg, and Lars Frelin
21. Gene Gun Immunization to Combat Malaria
Elke S. Bergmann-Leitner and Wolfgang W. Leitner
22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination
Toshi Nagata and Yukio Koide
23. Biolistic DNA Vaccination Against Trypanosoma Infection
Marianne Bryan, Siobhan Guyach, and Karen A. Norris
24. Biolistic DNA Vaccination Against Melanoma
Julia Steitz and Thomas Tuting
25. Biolistic DNA Vaccination Against Cervical Cancer
Michal Smahel
26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy
Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe
27. Safety Assessment of Biolistic DNA Vaccination
Barbara Langer, Matthias Renner, Jurgen Scherer, Silke Schule, and Klaus Cichutek
Part VI: Related Applications for Biolistic Delivery of Molecules
28. DiOlistics: Delivery of Fluorescent Dyes into Cells
Nyssa Sherazee and Veronica A. Alvarez
29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)
Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun
Meng-Chi Yen and Ming-Derg Lai
15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery
Ahmad Jalili
16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs
Steve Pascolo
17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter
Yvonne Hoehn, Stephan Sudowe, and Angelika B. Reske-Kunz
18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice
Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus
Part V: Biolistic DNA Vaccination in Animal Disease Models
19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets
Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller
20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models
Gustaf Ahlen, Matti Sallberg, and Lars Frelin
21. Gene Gun Immunization to Combat Malaria
Elke S. Bergmann-Leitner and Wolfgang W. Leitner
22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination
Toshi Nagata and Yukio Koide
23. Biolistic DNA Vaccination Against Trypanosoma Infection
Marianne Bryan, Siobhan Guyach, and Karen A. Norris
24. Biolistic DNA Vaccination Against Melanoma
Julia Steitz and Thomas Tuting
25. Biolistic DNA Vaccination Against Cervical Cancer
Michal Smahel
26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy
Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe
27. Safety Assessment of Biolistic DNA Vaccination
Barbara Langer, Matthias Renner, Jurgen Scherer, Silke Schule, and Klaus Cichutek
Part VI: Related Applications for Biolistic Delivery of Molecules
28. DiOlistics: Delivery of Fluorescent Dyes into Cells
Nyssa Sherazee and Veronica A. Alvarez
29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)
Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss
Mulpuri Sujatha and K.B.R.S. Visarada