Part I. Single Molecule Techniques
1. Immobilization of Proteins for Single Molecule Fluorescence Resonance Energy Transfer Measurements of Conformation and Dynamics
Ucheor B. Choi, Keith R. Weninger, and Mark E. Bowen
2. Application of Confocal Single Molecule FRET to Intrinsically Disordered Proteins
Benjamin Schuler, Sonja Muller-Spath, Andrea Sorranno, Daniel Nettles
3. Single-Molecule Force Spectroscopy of Chimeric Polyprotein Constructs Containing Intrinsically Disordered Domains
Marco Brucale, Isabella Tessari, Luigi Bubacco, Bruno Samori
4. Visualization of Mobility by Atomic Force Microscopy
Toshio Ando and Noriyuki Kodera
5. Unequivocal Single-Molecule Force Spectroscopy of Intrinsically Disordered Proteins
Javier Oroz, Ruben Hervas, Alejandro Valbuena & Mariano Carrion-Vazquez
Part II. Methods to Assess Protein Size and Shape
6. Sedimentation Velocity Analytical Ultracentrifugation for Intrinsically Disordered Proteins
Andres G. Salvay, Guillaume Communie, and Christine Ebel
7. Analysis of Intrinsically Disordered Proteins by Small-Angle X-ray Scattering
Pau Bernado and Dmitri I. Svergun
8. Small Angle Neutron Scattering (SANS) for the Structural Study of Intrinsically Disordered Proteins in Solution: a Practical Guide
Frank Gabel
9. Dynamic and Static Light Scattering of Intrinsically Disordered Proteins
Klaus Gast and Christian Fiedler
10. Estimation of Intrinsically Disordered Protein Shape and Time-averaged Apparent Hydration in Native Conditions by a Combination of Hydrodynamic Methods
Johanna C. Karst, Ana Cristina Sotomayor-Perez, Daniel Ladant and Alexandre Chenal
11. Size-Exclusion Chromatography in Structural Analysis of Intrinsically Disordered Proteins
Vladimir N. Uversky
Part III. Methods to Analyze Conformational Behavior
12. Denaturant-induced Conformational Transitions in Intrinsically Disordered Proteins
Paolo Neyroz, Stefano Ciurli, and Vladimir N. Uversky
13. Identification of Intrinsically Disordered Proteins by a Special 2D Electrophoresis
Agnes Tantos, Peter Tompa
14. pH-induced Changes in Intrinsically-disordered Proteins
Matthew D. Smith and Masoud Jelokhani-Niaraki
15. Temperature Induced Transitions in Disordered Proteins Probed by NMR Spectroscopy
Magnus Kjaergaard, Flemming M. Poulsen and Birthe B. Kragelund
16. Analyzing Temperature Induced Transitions in Disordered Proteins by NMR Spectroscopy and Secondary Chemical Shift Analyses
Magnus Kjaergaard, Flemming M. Poulsen & Birthe B. Kragelund
17. Osmolyte-, Binding-, and Temperature-Induced Transitions of Intrinsically Disordered Proteins
Allan Chris M. Ferreon and Ashok A. Deniz
18. Laser Temperature-jump Spectroscopy of Intrinsically Disordered Proteins
Stephen J. Hagen
19. Differential Scanning Microcalorimetry of Intrinsically Disordered Proteins
Sergei E. Permyakov
20. Identifying Disordered Regions in Proteins by Limited Proteolysis
Angelo Fontana, Patrizia Polverino de Laureto, Barbara Spolaore and Erica Frare
21. The Effect of Counter Ions on the Conformation of Intrinsically Disordered Proteins Studied by Size-exclusion Chromatography
Magdalena Wojtas, Tomasz M. Kaplon, Piotr Dobryszycki, Andrzej Ozyhar
22. Mean Net Charge of Intrinsically Disordered Proteins: Experimental Determination of Protein Valence by Electrophoretic Mobility Measurements
Ana Cristina Sotomayor-Perez, Johanna C. Karst, Daniel Ladant and Alexandre Chenal
23. Protein Characterization by Partitioning in Aqueous Two-Phase Systems
Larissa Mikheeva, Pedro Madeira, and Boris Zaslavsky
Part IV. Mass-Spectrometry
24. Detection and Characterization of Large-scale Protein Conformational Transitions in Solution using Charge-state Distribution Analysis in ESI-MS
Rinat R. Abzalimov, Agya K. Frimpong and Igor A. Kaltashov
25. Localizing Flexible Regions in Proteins using Hydrogen Deuterium Exchange Mass Spectrometry
Cedric E. Bobst and Igor A. Kaltashov
26. Mass-spectrometry Tools for Analysis of Intermolecular Interactions
Jared R. Auclair, Mohan Somasundaran, Karin M. Green, James E. Evans, Celia A. Schiffer, Dagmar Ringe, Gregory A. Petsko, and Jeffrey N. Agar
27. Characterization of Oligomerization- Aggregation Products of Neurodegenerative Target Proteins by Ion Mobility Mass Spectrometry
Camelia Vlad, Marius Ionut Iurascu, Stefan Slamnoiu, Bastian Hengerer, and Michael Przybylski
Part V. Expression and Purification of IDP's
28. Identifying Solubility-promoting Buffers for Intrinsically Disordered Proteins Prior to Purification
Kelly A. Churion and Sarah E. Bondos
29. Proteomic Methods for the Identification of Intrinsically Disordered Proteins
Agnes Tantos, Peter Tompa
30. Selective Isotope Labeling of Recombinant Proteins in E. coli
Kit I. Tong, Masayuki Yamamoto, and Toshiyuki Tanaka