1.Methodology For The High-Throughput Identification and Characterization of tRNA Variants that are Substrates for a tRNA Decay Pathway Matthew J. Payea, Michael P. Guy, and Eric M. Phizicky 2.Nucleoside Analysis by Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry Yuriko Sakaguchi, Kenjyo Miyauchi, Byeong-il Kang, and Tsutomu Suzuki 3.A Platform for Discovery and Quantification of Modified Ribonucleosides in RNA: Application to Stress-Induced Reprogramming of tRNA Modifications Weiling Maggie Cai, Yok Hian Chionh, Fabian Hia, Chen Gu, Stefanie Kellner, Megan E. McBee, Chee Sheng Ng, Yan Ling Joy Pang, Erin G. Prestwich, Kok Seong Lim, I. Ramesh Babu, Thomas J. Begley and Peter C. Dedon 4.Recognition of Specified RNA Modifications by the Innate Immune System Tatjana Eigenbrod, Patrick Keller, Steffen Kaiser, Katharina Rimbach, Alexander H. Dalpke and Mark Helm 5.Kinetic Analysis of tRNA Methyl Transferases Ya-Ming Hou and Isao Masuda 6.Preparation of Human Nuclear RNA m6A Methyltransferases and Demethylases and Biochemical Characterization of Their Catalytic Activity Jianzhao Liu, Yanan Yue, and Chuan He 7.Transcriptome-Wide Mapping of N6-Methyladenosine by m6A-Seq Dan Dominissini, Sharon Moshitch Moshkovitz, Ninette Amariglio and Gideon Rechavi 8.Probing RNA Modification Status at Single-Nucleotide Resolution in Total RNA Nian Liu and Tao Pan 9.High-resolution mapping of N6-methyladenosine in transcriptome and Genome Using a Photo-Crosslinking-Assisted Strategy Kai Chen, Guan-Zheng Luo and Chuan He 10.Pseudouridine in Mrna: Incorporation, Detection and Recoding Guowei Wu, Chao Huang and Yi-Tao Yu 11.Pseudo-Seq: Genome-Wide Detection of Pseudouridine Modifications in RNA T homas M. Carlile, Maria F. Rojas-Duran and Wendy V. Gilbert 12.Pseudouridine Chemical Labeling and Profiling Xiaoyu Li, Shiqing Ma and Chengqi Yi 12.Experimental Approaches for Target Profiling RNA Cytosine Methyltransferases Vahid Khoddami, Archana Yerra, and Bradley R. Cairns 13.RNA 5-Methylcytosine Analysis by Bisulfite Sequencing Matthias Schaefer 14.Biochemical and transcriptome-wide identification of A-to-I RNA editing sites by ICE-seq Shunpei Okada, Masayuki Sakurai, Hiroki Ueda, and Tsutomu Suzuki 15.Radical SAM-Mediated Methylation of Ribosomal RNA Vanja Stojkovic and Danica Galonic Fujimori