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Cardiovascular Development Xu Peng

Cardiovascular Development By Xu Peng

Cardiovascular Development by Xu Peng


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Summary

These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research.

Cardiovascular Development Summary

Cardiovascular Development: Methods and Protocols by Xu Peng

sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics.

Cardiovascular Development Reviews

From the reviews:

This is an up-to-date review of current research techniques used in the field of cardiovascular development. ... The book successfully instructs researchers on proper experimental techniques, giving step-by-step instructions, required for research in this field. ... this book is intended for use by both novice and experienced basic science researchers. ... This book is an excellent reference for any researcher in this area. (Patrick T. Campbell, Doody's Book Reviews, April, 2012)

Table of Contents

Preface

Contributors

Part I: Model organisms

1. Use of Whole Embryo Culture for Studying Heart Development

Calvin T. Hang, and Ching-Pin Chang

2. Quantifying Cardiac Functions in Embryonic and Adult Zebrafish

Tiffany Hoage, Yonghe Ding, and Xiaolei Xu

3. Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting

Ching-Pin Chang

4. Morpholino Injection in Xenopus

Panna Tandon, Chris Showell, Kathleen Christine, and Frank L Conlon

5. Chicken Chorioallantoic Membrane Angiogenesis Model

Domenico Ribatti

6. Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography

Christopher E. Schmitt, Melinda B. Holland, and Suk-Won Jin

7. Whole-mount Confocal Microscopy for Vascular Branching Morphogenesis

Yoh-suke Mukouyama, Jennifer James, Joseph Nam, and Yutaka Uchida

8. Visualization of Mouse Embryo Angiogenesis by Fluorescence-based Staining

Yang Liu, Marc Antoniyak, and Xu Peng

9. Miniaturized Assays of Angiogenesis in Vitro

May J. Reed and Robert B. Vernon

Part II: Cell and Molecular Biology Methods

10. Analysis of the Endocardial-to-Mesenchymal Transformation of Heart Valve Development by a Collagen Gel Culture Assay

Ching-Pin Chang, Yiqin Xiong, and Bin Zhou.

11. Quantification of Myocyte Chemotaxis: A role for FAK in Regulating Directional Motility

Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor

12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining

Yanping Zhang and L. Bruno Ruest

13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte

Jieli Li, Marc Antonyak, and Xu Peng

14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung

Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng

15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells

Bin Zhou and William T. Pu

16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization

Jan Patterson, Rick Metz, and Emily Wilson

17. C-kit Expression Identifies Cardiac Precursor Cells

Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny

18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols

Dongfei Qi and Mingui Fu

19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels

Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja

20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups

Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,

Xu Peng, and David E. Dostal

Part III: New Techniques

21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways

Shenyuan Zhang and Hongying Zheng

22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy

23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

Eric A. Bridenbaugh

25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

Vincent VanBuren

26. High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor

12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining

Yanping Zhang and L. Bruno Ruest

13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte

Jieli Li, Marc Antonyak, and Xu Peng

14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung

Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng

15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells

Bin Zhou and William T. Pu

16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization

Jan Patterson, Rick Metz, and Emily Wilson

17. C-kit Expression Identifies Cardiac Precursor Cells

Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny

18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols

Dongfei Qi and Mingui Fu

19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels

Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja

20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups

Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,

Xu Peng, and David E. Dostal

Part III: New Techniques

21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways

Shenyuan Zhang and Hongying Zheng

22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy

23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

Eric A. Bridenbaugh

25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

Vincent VanBuren

26. High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

Bin Zhou and William T. Pu

16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization

Jan Patterson, Rick Metz, and Emily Wilson

17. C-kit Expression Identifies Cardiac Precursor Cells

Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny

18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols

Dongfei Qi and Mingui Fu

19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels

Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja

20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups

Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,

Xu Peng, and David E. Dostal

Part III: New Techniques

21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways

Shenyuan Zhang and Hongying Zheng

22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy

23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

Eric A. Bridenbaugh

25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

Vincent VanBuren

26. High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy

23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

Eric A. Bridenbaugh

25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

Vincent VanBuren

26. High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal

24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis

Eric A. Bridenbaugh

25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research

Vincent VanBuren

26. High-speed Confocal Imaging of Zebrafish Heart Development

Michael P. Craig and Jay R. Hove

27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

Michael P. Craig and Jay R. Hove

27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays

David G. Taylor and Anupama Natarajan

David G. Taylor and Anupama Natarajan

Additional information

NLS9781493958535
9781493958535
1493958534
Cardiovascular Development: Methods and Protocols by Xu Peng
New
Paperback
Humana Press Inc.
2016-08-23
341
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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