Part I: Approaches to the Detection and Analysis of HPV Types
1. Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer
Zigui Chen, Luciana Bueno de Freitas, and Robert D. Burk
2. A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus
M. Isabel Micalessi, Gaelle A. Boulet, and J. Jean-Paul Bogers
Part II: Molecular Pathogenesis of CxCA
3. Replication of Human Papillomavirus in Culture
Eric J. Ryndock, Jennifer Biryukov, and Craig Meyers
4. HPV Binding Assay to Laminin-332/Integrin 6 4 on Human Keratinocytes
Sarah A. Brendle and Neil D. Christensen
5. Methods to Assess the Nucleocytoplasmic Shuttling of the HPV E1 Helicase and Its Effects on Cellular Proliferation and Induction of a DNA Damage Response
Michael Lehoux, Amelie Fradet-Turcotte, and Jacques Archambault
6. Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cycle
Jason M. Bodily
7. Robust HPV-18 Production in Organotypic Cultures of Primary Human Keratinocytes
Hsu-Kun Wang, Thomas R. Broker, and Louise T. Chow
8. A High-Throughput Cellular Assay to Quantify the p53-Degradation Activity of E6 from Different Human Papillomavirus Types
David Gagnon and Jacques Archambault
9. Retroviral Expression of Human Cystatin Genes in HeLa Cells
Crystal M. Diep, Gagandeep Kaur, Daniel Keppler, and Athena W. Lin
Part III: Vaccinations and Immune Responses to HPV Infections
10. Molecular Analysis of Human Papillomavirus Virus-Like Particle Activated Langerhans Cells In Vitro
Andrew W. Woodham, Adam B. Raff, Diane M. Da Silva, and W. Martin Kast
Part IV: Identification of Novel Potential Drug Targets
11. Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells
Peralta-Zaragoza Oscar, De la O-Gomez Faustino, Deas Jessica, Fernandez-Tilapa Gloria, Fierros-Zarate Geny del Socorro, Gomez-Ceron Claudia, Burguete-Garcia Ana, Torres-Poveda Kirvis, Bermudez-Morales Victor Hugo, Rodriguez-Dorantes Mauricio, Perez-Plasencia Carlos, and Madrid-Marina Vicente
12. Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells
Wenyi Gu, Nigel McMillan, and Chengzhong Yu
13. Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays
Gina J. Ferris, Lauren B. Shunkwiler, and Charles A. Kunos
14. Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe
Colleen Rivard and Martina Bazzaro
Part V: Approaches to Detecting, Typing, and Staging of CxCA
15. Immunocytochemical Analysis of the Cervical Pap Smear
Terry K. Morgan and Michelle Berlin
16. Diagnosis of HPV-Negative, Gastric-Type Adenocarcinoma of the Endocervix
Edyta C. Pirog
17. Targeting of the HPV-16 E7 Protein by RNA Aptamers
Julia Dolores Toscano-Garibay, Maria Luisa Benitez-Hess, and Luis Marat Alvarez-Salas
18. The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer
Cara M. Martin, Katharine Astbury, Louise Kehoe, Jacqueline Barry O'Crowley, Sharon O'Toole, and John J. O'Leary
19. Fixation Methods for the Preservation of Morphology, RNAs, and Proteins in Paraffin-Embedded Human Cervical Cancer Cell Xenografts in Mice
Yoko Matsuda and Toshiyuki Ishiwata
Part VI: Genomic Analyses of CxCA
20. Assessment of the HPV DNA Methylation Status in Cervical Lesions
Mina Kalantari and Hans-Ulrich Bernard
21. MeDIP-on-Chip for Methylation Profiling
Yaw-Wen Hsu, Rui-Lan Huang, and Hung-Cheng Lai
22. Use of DBD-FISH for the Study of Cervical Cancer Progression
Eva I. Cortes-Gutierrez, Jose L. Fernandez, Martha I. Davila-Rodriguez, Carmen Lopez-Fernandez, and Jaime Gosalvez
Part VII: Cellular Assays and Cell Culture Models of CxCA
23. A Quantitative and High-Throughput Assay of Human Papillomavirus DNA Replication
David Gagnon, Amelie Fradet-Turcotte, and Jacques Archambault
24. Native Human Papillomavirus Production, Quantification, and Infectivity Analysis
Jennifer Biryukov, Linda Cruz, Eric J. Ryndock, and Craig Meyers
25. Functional Analysis of HPV-Like Particle-Activated Langerhans Cells In Vitro
Lisa Yan, Andrew W. Woodham, Diane M. Da Silva, and W. Martin Kast
26. Assessment of the Radiation Sensitivity of Cervical Cancer Cell Lines
Mayil Vahanan Bose and Thangarajan Rajkumar
Part VIII: Animal Models of CxCA and Experimental Therapeutic Strategies
27. Mouse Model of Cervicovaginal Papillomavirus Infection
Nicolas Cuburu, Rebecca J. Cerio, Cynthia D. Thompson, and Patricia M. Day
28. Establishment of Orthotopic Primary Cervix Cancer Xenografts
Naz Chaudary, Karolina Jaluba, Melania Pintilie, and Richard P. Hill
29. Generation of K14-E7/ N87sscat Double Transgenic Mice as a Model of Cervical Cancer
Gulay Bulut and Aykut UEren