Airway Inflammation and Remodeling in Asthma: Current Concepts Stephen T. Holgate Part I. Sample Collection: Invasive Techniques Biopsy Techniques: Optimization for Collection and Preservatio Marina Saetta and Graziella Turato Bronchoalveolar Lavage (BAL): Critical Evaluation of Techniques Christopher Ward and E. Haydn Walters Nasal Secretions and Exudations: Collection and Approaches to Analysis Lennard Greiff, Morgan Andersson, and Carl G.A. Persson Part II. Sample Collection: Noninvasive Techniques Induced Sputum: Selection Method Margaret M. Kelly, Ann Efthimiadis, and Frederick E. Hargreave Induced Sputum: Whole Sample Vera M. Keatings and Julia A. Nightingale Measurement of Exhaled Nitric Oxide and Carbon Monoxide Sergei A. Kharitonov Measurement of Exhaled Hydrocarbons Paolo Paredi, Sergei A. Kharitonov, and Peter J. Barnes Breath Condensate as a Vehicle for Collection of Inflammatory Mediators, Especially Hydrogen Peroxide Johan C. de Jongste, Rijn Joebsis, and H. Rolien Raatgeep Part III. Cell Isolation and Culture Airway Epithelial Cells (Primaries vs Cell Lines) Louise E. Donnelly Isolation and Culture of Human Alveolar Type II Pneumocytes Ian R. Witherden and Teresa D. Tetley Pulmonary Artery Endothelial Cells Joachim Seybold and Norbert Suttorp Isolation and Culture of Human Airway Smooth Muscle Cells Aili L. Lazaar and Reynold A. Panettieri, Jr Isolation and Purification of Human Mast Cells and Basophils Bernhard F. Gibbs and Madeleine Ennis Neutrophils: Collection, Separation, and Activation Sarah V. Culpitt Macrophages: Identification, Separation, and Function Leonard W. Poulter and C.M. Burke Collection, Separation, and Activation of Human T Lymphocytes YannisSotsios and Stephen G. Ward Eosinophils: Collection, Separation, and Activation Leo Koenderman, Jan van der Linden, Laurien Ulfman, and Paul Coffer Part IV. Gene Expression/Regulation Analysis of Gene Expression: Reverse Transcription-Polymerase Chain Reaction El-Bdaoui Haddad and Jonathan Roussell Analysis of Gene Expression: Northern Blotting Jonathan Roussell and El-Bdaoui Haddad The Technique of In Situ Hybridization Sun Ying and A. Barry Kay Measurement of Airway Mucin Gene Expression Kelly Pritchard, Alinka K. Smith, and Duncan F. Rogers Transcription Factors and Inflammatory Gene Regulation: Strategic Approaches Robert Newton and Karl J. Staples Part V. Mediators: Expression and Function Inflammatory Mediators in Spontaneously Produced Sputum Adam Hill, Simon Gompertz, Darren Bayley, and Robert Stockley Flow Cytometry: Measurement of Leukocyte Receptor Expression and Function Adele Hartnell Measurement of Granulocyte Pharmacodynamics in Whole Blood by Flow Cytometry Shannon A. Bryan, Margaret J. Leckie, Gavin Jenkins, Peter J. Barnes, Timothy J. Williams, Ian Sabroe, and Trevor T. Hansel Current In Vitro Models of Leukocyte Migration: Methods and Interpretation Jennifer R. Allport, Guillermo Garcia-Cardena, Yaw-Chyn Lim, and Francis W. Luscinskas Tracing Intracellular Mediator Storage and Mobilization in Eosinophils Salahaddin Mahmudi-Azer, Paige Lacey, and Redwan Moqbel Measurement of Metalloproteinases Steven D. Shapiro, Diane Kelley and Dale Kobayashi Immunocytochemistry and Immunohistochemistry Jochen Springer and Axel Fischer The Measurement of Cysteinyl Leukotrienes in Urine Jay Y. Wescott and Sally E. Wenzel Quantitative Analysis of Cyclooxygenase Metabolites of Arachidonic Acid Ryszard