Part I: Analysis of Viral Nucleic Acid Sensing In-Silico and In-Vitro
1. Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
Gernot Nees, Andreas Kaufmann and Stefan Bauer
2. Enzymatic Synthesis and Purification of a Defined RIG-I Ligand
Marion Goldeck, Martin Schlee, Gunther Hartmann, and Veit Hornung
3. Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis
Filiz Civril and Karl-Peter Hopfner
4. Isolation of RIG-I-associated RNAs from Virus Infected Cells
Andreas Schmidt, Andreas Linder, Nicolas Linder, and Simon Rothenfusser
5. Structure Modeling of Toll-like Receptors
Jing Gong and Tiandi Wei
6. Nucleic Acid Recognition in Dendritic Cells
Alexander Heiseke, Katharina Eisenacher, and Anne Krug
7. Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems
Andrea Ribeiro and Markus Woernle
8. Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro
Simone Romoli, Adriana Migliorini
9. In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes
Saskia Ziegler and Isabelle Bekeredjian-Ding
10. Mapping of Optimal CD8 T Cell Epitopes
Julia Roider, Thomas Vollbrecht and Rika Draenert
11. A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium, and Viral Infection
Viktor H. Koelzer and David Anz
12. MicroRNA Methodology: Advances in miRNA-technologies
Theresa Kaeuferle, Sabine Bartel, Stefan Dehmel, and Susanne Krauss-Etschmann
Part II: Analysis of Nucleic Acid Sensing In-Vivo
13. Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR)
Maciej Lech and Hans-Joachim Anders
14. Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus
Amanda A. Watkins, Ramon G. B. Bonegio and Ian R. Rifkin
15. Induction and Analysis of Nephrotoxic Serum Nephritis in Mice
John M. Hoppe and Volker Vielhauer
16. Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice
Moritz Rapp, David Anz, and Max Schnurr
17. Bifunctional siRNAs for Tumor Therapy
Fanny Matheis and Robert Besch