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Antibody Engineering John McCafferty

Antibody Engineering By John McCafferty

Antibody Engineering by John McCafferty


£179.89
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Summary

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. The time is now ripe to assemble in a Practical Approach volume the protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantitites.

Antibody Engineering Summary

Antibody Engineering: A Practical Approach by John McCafferty

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities.

Antibody Engineering Reviews

This book is very useful for practising antibody engineers, providing extra information that enables them to adopt new techniques or expand into new areas. ... this is a useful and interesting resource for practising antibody engineers and I will make sure my students have access to it. * SGM Quarterly, August 1997 *

Table of Contents

1. Construction and use of antibody gene repertoires ; 2. Affinity maturation of antibodies using phage display ; 3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs ; 4. Measuring antibody affinities in solution ; 5. Measuring antibody affinities using Bio-sensors ; 6. Analysis of human antibody sequences ; 7. Rodent to human antibodies by CDR grafting ; 8. Rodent to human antibodies by phage display ; 9. Choosing and manipulating effector functions ; 10. Secretion and purification of antibody fragments expressed in bacteria ; 11. Production of single chain Fv monomers and multimers ; 12. Expression of immunoglobulin genes in mammalian cells ; 13. In vitro linkage of antibody fragments into dimeric and multimeric forms

Additional information

NLS9780199635924
9780199635924
0199635927
Antibody Engineering: A Practical Approach by John McCafferty
New
Paperback
Oxford University Press
1996-06-27
348
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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Customer Reviews - Antibody Engineering