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Concepts and Techniques in Genomics and Proteomics N Saraswathy

Concepts and Techniques in Genomics and Proteomics By N Saraswathy

Concepts and Techniques in Genomics and Proteomics by N Saraswathy

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Concepts and Techniques in Genomics and Proteomics Summary

Concepts and Techniques in Genomics and Proteomics by N Saraswathy

Concepts and techniques in genomics and proteomics covers the important concepts of high-throughput modern techniques used in the genomics and proteomics field. Each technique is explained with its underlying concepts, and simple line diagrams and flow charts are included to aid understanding and memory. A summary of key points precedes each chapter within the book, followed by detailed description in the subsections. Each subsection concludes with suggested relevant original references.

About N Saraswathy

Dr Nachimuthu Saraswathy is currently Associate Professor at the Department of Biotechnology, Kumaraguru College of Technology. She teaches courses on Molecular Biology, Genetic Engineering, and Plant Biotechnology, as well as Genomics and Proteomics. Dr Saraswathy gained her Ph.D. in Molecular Biology from the National Centre for Plant Biotechnology, New Delhi. She has published several articles in journals of biotechnology and biochemistry. Mr Ponnusamy Ramalingam is Associate Professor at the Department of Biotechnology, Kumaraguru College of Technology. He received his M.Sc. In Biochemistry from Gulbarga University and Mtech in Biotechnology from Anna University. He has also taught and researched in institutions such as Kongu Engineering College and PNC&KR College, and has contributed several published journal articles.

Table of Contents

List of figures List of tables Preface Acknowledgements List of abbreviations About the authors Chapter 1: Introduction to genes and genomes Abstract: 1.1 Introduction 1.2 The cell 1.3 Mendel's contributions 1.4 The chromosomal theory of inheritance 1.5 The chemical nature of genetic material 1.6 Composition and structure of DNA 1.7 The central dogma of life 1.8 Genomes of prokaryotes and eukaryotes 1.9 The molecular structure of the gene 1.10 Conclusion Review questions and answers Web addresses Chapter 2: The human genome project Abstract: 2.1 The history of the HGP 2.2 The budget for the HGP 2.3 Goals of the HGP 2.4 Laboratories and investigators involved in the HGP 2.5 The Human Genome Organization (HUGO) 2.6 Salient findings of the HGP 2.7 Potential applications of the HGP 2.8 Post-HGP challenges 2.9 Ethical, legal, social issues (ELSI) related to the HGP 2.10 The international HapMap Project Review questions and answers Web addresses Chapter 3: Genomes of model organisms Abstract: 3.1 Introduction 3.2 The viral genome 3.3 Bacterial genomes 3.4 Fungal genomes 3.5 Worm genome: Caenorhabditis elegans 3.6 Fruit fly: Drosophila melanogaster 3.7 Plant genome 3.8 Animal genome 3.9 The Microbial Genome Project Chapter 4: High capacity vectors Abstract: 4.1 Introduction 4.2 Cosmid vectors 4.3 Fosmid vectors 4.4 Bacteriophage P1 derived vector 4.5 P1 derived artificial chromosome (PAC) 4.6 Bacterial artificial chromosomes (BAC) 4.7 Yeast artificial chromosome (YAC) Review questions and answers Web address Chapter 5: DNA sequencing methods Abstract: Key concepts 5.1 The history of DNA sequencing 5.2 Steps in DNA sequencing 5.3 Chemical degradation method of DNA sequencing 5.4 The chain termination method of DNA sequencing 5.5 Advances in DNA sequencing methods 5.6 New sequencing methods 5.7 Next generation sequencing methods Web addresses Chapter 6: Genome mapping Abstract: Key concepts 6.1 Introduction 6.2 Importance of genome mapping in the context of genome sequencing 6.3 Genetic mapping 6.4 Genetic mapping in humans 6.5 Physical mapping methods Chapter 7: Genome sequencing methods Abstract: 7.1 Introduction 7.2 The clone-by-clone genome sequencing method 7.3 The whole genome shotgun sequencing method 7.4 Error control in genome sequencing Chapter 8: Genome sequence assembly and annotation Abstract: 8.1 Introduction 8.2 Clone contig assembly 8.3 Genome assembly program 8.4 Gaps and gap closing methods 8.5 Draft and finished genome sequences 8.6 Genome annotation 8.7 Comparative genomics Chapter 9: Functional genomics Abstract: Key concepts 9.1 Introduction 9.2 Northern blotting 9.3 Subtractive hybridization 9.4 Differential Display Reverse Transcription PCR (DDRT-PCR) 9.5 Representational Difference Analysis (RDA) 9.6 Serial Analysis Gene Expression (SAGE) 9.7 Microarray technology Web addresses Chapter 10: Introduction to proteomics Abstract: Key concepts 10.1 Introduction 10.2 Traditional route of protein study 10.3 Protein isolation methods 10.4 Branches of proteomics 10.5 Characteristics of proteomics Chapter 11: Two-dimensional gel electrophoresis of proteins Abstract: 11.1 Introduction 11.2 Principles of 2D-PAGE 11.3 2D-PAGE apparatus 11.4 Sample preparation 11.5 First-dimensional separation by isoelectric focusing 11.6 Equilibration 11.7 Second-dimensional separation by SDS-PAGE 11.8 Detection of proteins on 2D-PAGE gels 11.9 Image analysis 11.10 Application of 2D-PAGE in proteomics Chapter 12: Mass spectrometry for proteomics Abstract: 12.1 Introduction 12.2 History of the mass spectrometer 12.3 Mass spectrometer 12.4 Protein sample preparation for MS analysis 12.5 Applications of MS proteomics Chapter 13: Protein Identification by Peptide Mass Fingerprinting (PMF) Abstract: Key concepts 13.1 Introduction 13.2 Principles of peptide mass fingerprinting 13.3 Protein preparation for PMF 13.4 Mass spectrometric analysis of peptide fragments 13.4 Data analysis and identification of protein Web address Chapter 14: Protein sequencing techniques Abstract: 14.1 Introduction 14.2 Preparation of protein sample for sequencing 14.3 Steps in protein sequencing 14.4 Protein sequencing by Edman degradation 14.5 De novo protein sequencing by mass spectrometry Review questions and answers Chapter 15: Phosphoproteomics Abstract: 15.1 Post-translational modifications of proteins 15.2 Phosphoproteomics 15.3 Phosphoprotein enrichment methods 15.4 Mass spectrometry for phosphoprotein identification Review questions and answers Chapter 16: Glycoproteomics Abstract: 16.1 Glycoproteins 16.2 Glycoprotein enrichment methods 16.3 Mass spectrometric analysis of glycoproteins 16.4 Importance of glycoproteins in human diseases Review questions and answers Conclusion Glossary Index

Additional information

NLS9780081017302
9780081017302
0081017308
Concepts and Techniques in Genomics and Proteomics by N Saraswathy
N Saraswathy
Woodhead Publishing Series in Biomedicine
New
Paperback
Elsevier Science & Technology
2016-08-19
268
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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