Part I: Overview of Microglia Biology
1. A Brief Overview of Multi-Talented Microglia
Bertrand Joseph and Jose Luis Venero
Part II: Isolation and Culture of Microglia
2. Cell Culturing of Human and Murine Microglia Cell Lines
Johanna Rodhe
3. Microglia Isolation from Adult Mouse Brain
Jae-Kyung Lee, Malu G Tansey
4. Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains
Tomas Deierborg
5. Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation Technology
Ashley S. Harms and Malu G. Tansey
6. Isolation and Culture of Adult Human Microglia within Mixed Glial Cultures for Functional Experimentation and High Content Analysis
Amy M. Smith, Hannah M. Gibbons, Claire Lill, Richard L. M. Faull and Mike Dragunow
Part III: Depletion and Transduction of Microglia
7. Depletion of Microglia from Primary Cellular Cultures
Lorena Pont-Lezica, Sabrina Colasse and Alain Bessis
8. Lentiviral Transduction of Cultured Microglia
Takahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh and Kazuhide Inoue
Part IV: Analysis of Microglial Cytokine Production
9. Microglial Activation: Measurement of Cytokines by Flow Cytometry
Deepak Kumar Kaushik and AnirbanBasu
10. In situ Hybridization of Cytokine mRNA using Alkaline Phosphatase Labelled Oligodeoxynucleotide Probes
Bettina Clausen, Christina Fenger and Bente Finsen
11. Use of Meso-scale DiscoveryTM to Examine Cytokinecontentin Microglia Cell Supernatant
Miguel A. Burguillos
Part V: Analysis of Microglia Activation
12. Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species
UrteNeniskyte and Guy C. Brown
13. Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells
Edel Kavanagh
14. Quantification of Microglial Phagocytosis by a Flow Cytometer-based Assay
Refik Pul, Chittappen Kandiyil Prajeeth, Martin Stangel
15. Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry
Alicia A. Babcock, Martin Wirenfeldt, and Bente Finsen
16. Fluorescence Imaging of Intracellular Ca2+, Na+ and H+ in Cultured Microglia
Tom Schilling, and Claudia Eder
17. Patch Clamp Protocols to Study Ion Channel Activity in Microglia
Tom Schilling, and Claudia Eder
Part VI: Analysis of Microglia Polarization18. Studying M1 and M2 States in Adult Microglia
Sadanand M. Gaikwad and Michael T. Heneka
19. Isolating, Culturing, and Polarizing Primary Human Adult and Fetal Microglia
Bryce A. Durafourt, Craig S. Moore, Manon Blain, and Jack P. Antel
Part VII: Co-culture Systems to Analysis Microglia Interactions with Other Cell Types
20. Understanding Microglia-neuron Cross-talk: Relevance of the Microglia-neuron Co-cultures
Fernando G Correa, Miriam Hernangomez, and Carmen Guaza
21. Preparation of Rodent Primary Cultures for Neuron-Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with Microglia
Shih-Heng Chen, Esteban A. Oyarzabal and Jau-Shyong Hong
Part VIII: Analysis of Microglia Functions in vivo
22. Microglia Detection by Enzymatic Histochemistry
Beatriz Almolda, Berta Gonzalez and Bernardo Castellano
23. Tomato Lectin Histochemistry for Microglial Visulization
Nadia Villacampa, Beatriz Almolda, Berta Gonzalez and Bernardo Castellano
24. Immunohistochemical Detection of Microglia
Rocio M. de Pablos, Ana M. Espinosa-Oliva, and Antonio J. Herrera
25. Intrathecal Infusion of Microglia Cells
Takahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh and Kazuhide Inoue
26. Intracranial Injection of LPS in Rat as Animal Model of Neuroinflammation
Ana M. Espinosa-Oliva, Rocio M. de Pablos, and Antonio J. Herrera
27. Analyses of Microglia Effector Function Using CX3CR1-GFP Knock In Mice
Jenny A. Garcia, Sandra M. Cardona and Astrid E. Cardona
28. In vivo Two-photon Microscopy of Microglia
Satoru Kondo and Shigeo Okabe
29. Use of Confocal Microscopy in the Study of Microglia in a Brain Metastasis Model
Manuel Sarmiento