Part I General Issues in Clinical Microbiology* General issues and role of laboratorians* Laboratory safety* Laboratory physical design, management, and organization* Quality in the clinical microbiology laboratory* Infection controlPart II Scientific and Laboratory Basis for Clinical Microbiology* Microbial taxonomy* Bacterial genetics, metabolism, and structure* Host-microorganism interactions* Role of microscopy in the diagnosis of infectious diseases* Laboratory cultivation and isolation of bacteria* Overview of conventional methods for bacterial identification* Molecular methods for microbial identification and characterization* Immunochemical methods used for organism detection* Serologic diagnosis of infectious diseases* Principles of antimicrobial action and resistance* Laboratory methods for detection of antimicrobial resistance* Laboratory strategies for antimicrobial susceptibility testingPart III Bacteriology* Overview of bacterial identification methods and strategies* Section 1: Catalase-positive, gram-positive cocci*Staphylococcus, Micrococcus, and similar organisms* Section 2: Catalase-negative, gram-positive cocci*Streptococcus, Enterococcus, and similar organisms* Section 3: Non-branching, catalase-positive, gram-positive bacilli*Bacillus and similar organisms*Listeria, Corynebacterium, and similar organisms* Section 4: Non-branching, catalase-negative, gram-positive bacilli*Erysipelothrix, Lactobacillus, and similar organisms * Section 5: Branching or partially acid-fast, gram-positive bacilli*Nocardia, Streptomyces, Rhodococcus, Oerskovia, and similar organisms* Section 6: Gram-negative bacilli and coccobacilli (MacConkey-positive, oxidase-negative)*Enterobacteriaceae*Acinetobacter, Chryseomonas, Flavimonas, and Stenotrophomonas* Section 7: Gram-negative bacilli and coccobacilli (MacConkey-positive, oxidase-positive)*Pseudomonas, Burkholderia, and similar organisms*Achromobacter, Agrobacterium, Ochrobactrum, and similar organisms*Chryseobacterium, Sphingobacterium, and similar organisms*Alcaligenes, Bordetella (nonpertussis), Comamonas, and similar organisms*Vibrio, Aeromonas, Plesiomonas, shigelloides, and Chromobacterium violaceum* Section 8: Gram-negative bacilli and coccobacilli (MacConkey-negative, oxidase-positive)*Sphingomonas paucimobilis and similar organisms*Moraxella and elongated Neisseria*Eikenella corrodens and similar organisms*Pasteurella and similar organisms *Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga and similar organisms* Section 9: Gram-negative bacilli and coccobacilli (MacConkey-negative, oxidase-variable)*Haemophilus* Section 10: Gram-negative bacilli that are optimally recovered on special media*Bartonella and Afipia*Campylobacter, Arcobacter, and Helicobacter*Legionella*Brucella*Bordetella pertussis and Bordetella parapertussis*Francisella*Streptobacillus moniliformis and Spirillum minus* Section 11: Gram-negative cocci*Neisseria and Moraxella catarrhalis* Overview and general considerations* Section 12: Anaerobic bacteriology* Laboratory considerations* Section 13: Mycobacteria and other bacteria with unusual growth requirements* Mycobacteria* Obligate intracellular and nonculturable bacterial agents* Cell wall-deficient bacteria: Mycoplasma and Ureaplasma* The spirochetesPart IV Parasitology* Laboratory methods for diagnosis of parasitic infectionsPart V Mycology* Laboratory methods in basic mycologyPart VI Virology * Laboratory methods in basic virologyPart VII Diagnosis by Organ System* Bloodstream infections* Infections of the lower respiratory tract* Upper respiratory tract infections and other infections of the oral cavity and neck* Meningitis and other infections of the central nervous system* Infections of the eyes, ears, and sinuses* Infections of the urinary tract* Genital tract infections* Gastrointestinal tract infections* Skin, soft tissue, and wound infections* Normally sterile body fluids, bone and bone marrow, and solid tissuesGlossary