1. Assessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary Microglia
Mareike Schnaars, Hannes Beckert, and Annett Halle
2. Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPP
Clara Westwell-Roper, Aisling Dunne, Man-Lyang Kim, C. Bruce Verchere, and Seth L. Masters
3. Assessment and Quantification of Crystal-induced Lysosomal Damage
Peter Duewell and Eicke Latz
4. Assessment of Inflammasome Activation in Primary Human Immune Cells
Theo S. Plantinga, Leo A.B. Joosten, and Mihai G. Netea
5. NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate Adjuvants
Marie Yang, Claire H.A. Hearnden, Ewa Oleszycka and Ed C. Lavelle
6. Measuring Inflammasome Activation in Response to Bacterial Infection
Petr Broz and Denise M. Monack
7. Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity
Manira Rayamajhi, Yue Zhang, and Edward A. Miao
8. ASC Speck Formation as a Read-out for Inflammasome Activation
Andrea Stutz, Gabor L. Horvath, Brian G. Monks, and Eicke Latz
9. Immunoblotting for Active Caspase-1
Christopher Jakobs, Eva Bartok, Andrej Kubarenko, Franz Bauernfeind and Veit Hornung
10. Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1 , IL-1 and Caspase-1
Katharina S. Schneider1, Christina J. Thomas, and Olaf Gross
11. Reconstituting the NLRP1 Inflammasome in vitro
Benjamin Faustin and John C. Reed
12. Assessing ATP Binding and Hydrolysis by NLR Proteins
Jinyao Mo and Joseph A. Duncan
13. Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition
Eric M. Kofoed and Russell E. Vance
14. Analysis of Microbiota Alterations in Inflammasome-deficient Mice
Eran Elinav, Christoph A Thaiss, and Richard A Flavell
15. Quantification of Adipose Tissue Leukocytosis in Obesity
Ryan Grant, Yun-Hee Youm, Anthony Ravussin, and Vishwa Deep Dixit
16. In vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates
Tamiko Yanagida, Kaoru Orihashi and Hajime Kono