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Handbook of Endocrine Research Techniques Flora de Pablo (Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain)

Handbook of Endocrine Research Techniques By Flora de Pablo (Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain)

Handbook of Endocrine Research Techniques by Flora de Pablo (Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain)

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Summary

Features methods and strategies useful in endocrinological research. Serving as a resource for both the novice and experienced investigator, this book includes chapters which provide an introduction to the area, general concepts, protocols and references.

Handbook of Endocrine Research Techniques Summary

Handbook of Endocrine Research Techniques by Flora de Pablo (Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain)

Written by experts in the field, Handbook of Endocrine Research Techniques is currently the only single source of up-to-date methods and strategies particularly useful in endocrinological research. As a resource for both the novice and experienced investigator, the book includes chapters which provide an introduction to the area, general concepts, detailed protocols, and extensive references.

About Flora de Pablo (Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain)

Colin G. Scanes was formerly editor of the journal Poultry Science and has held senior faculty and/or administrative positions at the University of Leeds, Rutgers-the State University of New Jersey, Iowa State University, Mississippi State University, and University of Wisconsin, Milwaukee. Dr. Scanes was also the editor for the latest (sixth) edition of Sturkie's Avian Physiology for Elsevier/Academic Press. He has extensive experience in teaching undergraduates and research with more than 600 publications.

Table of Contents

Hormone Assays. Radioimmunoassay (RIA): Concept and Types. Immunoradiometric (IRMA) Assays. Enzyme-Linked Immunoabsorbent Assays (ELISA). Development and Characterization of Polyclonal and Monoclonal Antibodies. Isolation of Polypeptide Hormones. Reverse Hemolytic Plaque Assays. Capillary Electrophoresis Coupled to Fluorescence Detection for the Determination of Multiple Neuropeptides. Immunoreactive Heterogeneity in Peptide Measurement. Why Use a Flow Cytometer for Endocrine Cell Analysis? Identification and Characterization of Insulin-Like Growth Factor Binding Proteins. Histochemical and in Situ Approaches. Localization of Peptides. Electron Microscopic Immunocytochemical Approaches to the Localization of Ligands, Receptors, Transducers, And Transporters. In Situ Hybridization for Hormone and Receptor Expression. Techniques for Receptors and Signal Transductors. Plasma Membrane Isolation Strategies for Cell Surface Receptors. Tyrosine Kinase Receptors. Involvement of Protein Tyrosine Phosphatase Activity in the Regulation of Insulin's Signal Transduction. Techniques for Evaluating Protein Kinase C in Intact Cells. Identification and Quantification of G Proteins. Determination of Adenylyl Cyclase Catalytic Activity. Intracellular Mediators of Peptide Hormone Action. Free Calcium Measurements. Molecular Techniques and Specific Model Systems. Approaches for the Purification, Quantitation, And Interpretation of Hormone and Receptor Mrnas. The Use of PCR in Endocrine Research. Detection of Mutations in Hormone Receptor Genes. Techniques to Study Regulation of Gene Expression. Transgenic Animals. The Use of Non-Mammalian Models. Proliferation Induced By Growth Factors in Mammalian Fetal Cells. Analysis of Hormones and Receptors in Embryogenesis.

Additional information

NPB9780122099205
9780122099205
0122099206
Handbook of Endocrine Research Techniques by Flora de Pablo (Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain)
Flora de Pablo (Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain)
New
Hardback
Elsevier Science Publishing Co Inc
1993-12-15
599
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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