Preface...
Table of Contents...
Contributing Authors...
1. Clearance of Maternal RNAs: Not a Mummy's Embryo Anymore
Antonio Marco
2. Link of Zygotic Genome Activation and Cell Cycle Control
Boyang Liu and Joerg Grosshans
3. Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis
Alessandro Rosa and Ali H. Brivanlou
4. Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR
Kyeoung-Hwa Kim, Su-Yeon Lee, and Kyung-Ah Lee
5. Detection of miRNA in Mammalian Oocytes and Embryos
Malavika K. Adur, Benjamin J. Hale, and Jason W. Ross
6. Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method
Nobuhiko Hamazaki, Kinichi Nakashima, Katsuhiko Hayashi, and Takuya Imamura
7. Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation
Jesus Garcia-Lopez, Eduardo Larriba, and Jesus del Mazo
8. Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
Patricia Heyn and Karla M. Meugebauer
9. RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos
Noemie Ranisavljevic, Ikuhiro Okamoto, Edith Heard, and Katia Ancelin
10. Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry
Irina O. Bogolyubova and Dmitry S. Bogolyubov
11. Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo
Yan Li and Christopher O'Neill
12. Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos
Ka Yi Ling, Lih Fenh Cheow, Stephen R. Quake, William F. Burkholder, and Daniel M. Messerschmidt
13. Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation
Kyungjun Uh and Kiho Lee
14. Targeted Gene Knockdown in Early Embryos using siRNA
Lu Zhang and Zoltan Machaty
15. Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput
Wenbo Wang, Yingfan Zhang, and Haoyi Wang
16. CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine
Junghyun Ryu and Kiho Lee
17. Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development
Veronika Benesova, Veronika Kinterova, Jiri Kanka, and Tereza Toralova
18. Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes
Keisuke Aoshima, Takashi Kimura, and Yuki Okada
