Microbiology Experiments: A Health Science Perspective by Kleyn Et Al
All experiments are correlated to Nester's Microbiology: A Human Perspective, 8e, but can be used with any non-majors/allied health microbiology textbook.
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Basic Microbiology
Introduction to Microbiology
1. Ubiquity of Microorganisms 2. Pure Culture and Aseptic Technique 3. Introduction to the Compound Light Microscope 4. The Oil Immersion LensIntroduction to Staining of Microorganisms
5. Simple Stains: Positive and Negative Stains 6. Differential and Other Special StainsIntroduction to Microbial Growth
7. Chemically Defined, Complex, Selective, and Differential Media 8. Quantification of MicroorganismsIntroduction to the Environment and Microbial Growth
9. Aerobic and Anaerobic Growth 10. The Effect of Incubation Temperature on Generation TimeIntroduction to Control of Microbial Growth
11. Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time 12. Control of Microbial Growth with Ultraviolet Light 13. Changes in Osmotic Pressure Due to Salt and Sugar and Their Effects on Microbial Growth 14. Antibiotics 15. Antiseptics and DisinfectantsIntroduction to Microbial Genetics
16. Selection of Bacterial Mutants Resistant to Antibiotics 17. Transformation: A Form of Genetic Recombination 18. Gene Regulation: Induction and Catabolite Repression PART TWOThe Other Microbial World
Introduction to the Other Microbial World
l9. Microscopic Identification of Fungi 20. Parasitology: Protozoa and Helminths 21. Titering Prokaryotic Viruses PART THREEMicrobes and Humans
Introduction to Medical Microbiology
22. Normal Skin Biota 23. Streptococci and Respiratory Microorganisms 24. Identification of Enteric Gram-Negative Rods 25. Clinical Unknown Identification PART FOURImmunology
Introduction to Some Immunological Principles and Techniques
26. Differential White Blood Cell Stains 27. Lysozyme, an Enzyme Contributing to Innate Immunity 28. Enzyme-Linked Immunoabsorbent Assay (ELISA) PART FIVEPublic Health
Introduction to Public Health
29. Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques 30. Microbial Examination of Food31. Epidemiology PART SIXApplications of Biotechnology
Introduction to Biotechnology
32. Identifying DNA with Restriction Enzymes and the Polymerase Chain Reaction (PCR) 33. Electrophoresis 34. Identification of Bacteria Using the Ribosomal Database Project 35. Microarray PART SEVENIndividual Projects
Introduction to the Individual Projects
36. Luminescent Bacteria: Bacteria That Produce Light APPENDICES1. Culture Media and Reagent Formulas 2. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in This Manual 3. Dilution Practice Problems 4. Alternative Procedures 5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size 6. History and Working Principles of Light Microscopy