Preface
Section 1 In vitro techniques
Chapter 1: In vitro angiogenesis assay - endothelial migration, proliferation, and tube formation
Chapter 2: Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis
Chapter 3: Induction of hypoxia in vascular endothelial cell culture
Chapter 4: Evaluating In Vitro Angiogenesis using Live Cell Imaging
Chapter 5: Isolation of Endothelial Progenitor Cells (EPCs)
Chapter 6: Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis
Chapter 7: Assessment of vascular function and contractility, ex vivo.
Chapter 8: Measurement of A uptake by cerebrovascular smooth muscle cells
Chapter 9: Measurement of Intracellular Ca2+ in Human Endothelial cells
Section 2: In vivo and ex vivo manipulations
Chapter 10: Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion
Chapter 11: Sponge implant model of inflammatory angiogenesis
Chapter 12: The chick embryo chorioallantoic membrane assay
Chapter 13: Dorsal air sac assay
Chapter 14: Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals
Chapter 15: Hypoxia-induced retinal angiogenesis in adult zebrafish
Chapter 16: Angiogenesis in regenerating adult zebrafish tail fin
Chapter 17: Methods for studying developmental angiogenesis in zebrafish
Chapter 18: Isolation and Expansion of Brain Microvascular Endothelial Cells
Section 3: Imaging and histological analysis
Chapter 19: Adipose angiogenesis
Chapter 20: Assessing tumor angiogenesis in histological samples
Chapter 21: Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea.
Chapter 22: Computed Tomography Angiography: Fundamental techniques and data interpretation
Chapter 23: Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation
Chapter 24: Single-Photon Emission Tomography of the brain in vascular pathology
Section 4: Miscellaneous novel techniques in vascular biology
Chapter 25: Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues
Chapter 26: Preparation of liposomes with dual fluorophores to follow real-time content release in vivo
Chapter 27: Vascular Flow Modelling using Computational Fluid Dynamics
Chapter 28: Reverse Transcription Real-Time PCR Protocol for gene expression analyses
Chapter 29: Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis
Chapter 30: Genomic microarray analysis
Chapter 31: Selection of appropriate housekeeping genes for quality control
Chapter 32: Endothelial transcriptomic analysis
Chapter 33: Protocol for Multiplex Amplicon Sequencing using Barcoded Primers
Chapter 34: Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells: Identification and Quantification
Chapter 35: A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood
Chapter 36: Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry