Part I. Neutrophils: an Overview
1. The Role of Neutrophils in the Immune System: An Overview
Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn
Part II. Neutrophil Isolation and Subcellular Fractionation
2. Isolation of Human Neutrophils
William M. Nauseef
3. Neutrophil Isolation from Non-Human Species
Daniel W. Siemsen, Natalia Malachowa, Igor A. Schepetkin, Adeline R. Whitney, Lilya N. Kirpotina, Benfang Lei, Frank R. DeLeo, and Mark T. Quinn
4. Collection of In vivo Transmigrated Neutrophils from Human Skin
Karin Christenson, Lena Bjoerkman, Lisa Davidsson, Anna Karlsson, Per Follin, Claes Dahlgren, and Johan Bylund
5. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
Stine Novrup Clemmensen, Lene Udby, and Niels Borregaard
Part III. Biochemistry, Biology and Signal Transduction of Neutrophils
6. Rho Family and Rap GTPase Activation Assays
Richard T. Jennings and Ulla G. Knaus
7. Measurement of Phospholipid Metabolism in Intact Neutrophils
Susan Sergeant and Linda C. McPhail
8. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium Signals in Neutrophils
Maurice B. Hallett , Maha Al-Jumaa, and Sharon Dewitt
9. Analysis of Electrophysiological Properties and Responses of Neutrophils
Deri Morgan and Thomas E. DeCoursey
10. Analysis of Neutrophil Apoptosis
David A. Dorward, Adriano G. Rossi, Ian Dransfield, and Christopher D. Lucas
11. Microinjection Methods for Neutrophils
Iraj Laffafian, Kimberly J. Lewis, K. Benjamin Masterman, and Maurice B. Hallett
12. Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells
Colin L. Sweeney, Randall K. Merling, Uimook Choi, Debra Long Priel, Douglas B. Kuhns, Hongmei Wang, and Harry L. Malech
Part IV. Neutrophil Adhesion and Chemotaxis
13. Neutrophil Migration Through Extracellular Matrix
Richard T. Jennings and Ulla G. Knaus
14. Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish
Pui-ying Lam, Robert S. Fischer, William D. Shin, Clare M. Waterman, and Anna Huttenlocher
15. Detection of Bi-Directional Signalilng During Integrin Activation and In Neutrophil Adhesion
Stuart M. Altman, Neha Dixit, and Scott I. Simon
Part V. Neutrophil Phagocytosis and Bactericidal Activity
16. Immunofluorescence and Confocal Microscopy of Neutrophils
Lee-Ann H. Allen
17. Expression of Genetically-Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils
Benjamin E. Steinberg, Marco A.O. Magalhaes, and Sergio Grinstein
18. Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry
Pontus Nordenfelt
19. Analysis of Neutrophil Bactericidal Activity
Heather A. Parker, Nicholas J. Magon, Jessie N. Green, Mark B. Hampton, and Christine C. Winterbourn
20. Induction and Quantification of Neutrophil Extracellular Traps
Alejandro Sanchez Gonzalez, Bart W. Bardoel, Christopher J. Harbort, and Arturo Zychlinsky
Part VI. NADPH Oxidase and Production of Reactive Oxygen Species
21. Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes
Johan Bylund, Halla Bjoernsdottir, Martina Sundqvist, Anna Karlsson, and Claes Dahlgren
22. Cell-free NADPH Oxidase Assays: In vitro Veritas
Edgar Pick
23. Assessment of Priming of the Human Neutrophil Respiratory Burst
Margarita Hurtado-Nedelec, Karama Makni-Maalej, Marie-Anne Gougerot-Pocidalo, Pham My-Chan Dang, and Jamel El-Benna
24. Affinity Purification and Reconstitution of Human Phagocyte Flavocytochrome b for Detection of Conformational Dynamics in the Membrane
Marcia Riesselman and Algirdas J. Jesaitis
25. Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies
Sahra Amel Belambri, Pham My-Chan Dang, and Jamel El-Benna
Part VII. Analysis of Neutrophil Gene Expression and Transcription Factors
26. Genome-Scale Transcript Analyses with Human Neutrophils
Scott D. Kobayashi, Daniel E. Sturdevant, and Frank R. DeLeo
27. Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils
Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni
28. High Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis
Flavia S. Lakschevitz and Michael Glogauer
29. Detection of Intact Transcription Factors in Human Neutrophils
Patrick P. McDonald and Richard D. Ye
Part VIII. Neutrophil Defects and Diagnosis
30. Disorders of Neutrophil Function: An Overview
Mary C. Dinauer
31. Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders
Houda Zghal Elloumi and Steven M. Holland
32. Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency
William M. Nauseef