Part I: Targeting Cancer Cell Metabolism as Anticancer Therapy
1. Use of Biguanides to Improve Response to Chemotherapy
Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner
2. Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells Via Metabolic Measurement and Clonogenic Survival
Sang Hyeok Woo, Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner
3. Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy
Nariman Ansari, Stefanie Muller, Katharina Hoette, Stefanie Rakel, Patrick Antonietti, Donat Koegel, Ernst H.K. Stelzer, and Francesco Pampaloni
Part II: Epigenetic Control of Cancer
4. Interdependency Between Genetic and Epigenetic Regulatory Defects in Cancer
Felix Recillas-Targa
5. Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF
Edgar Gonzalez-Buendia, Rosario Perez-Molina, Erandi Ayala-Ortega, Georgina Guerrero, and Felix Recillas-Targa
6. Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers
Fermin Mar-Aguilar, Cristina Rodriguez-Padilla, and Diana Resendez-Perez
7. Prostate Cancer Detection Using a Non-Invasive Method for Quantifying miRNAs
Mauricio Rodriguez-Dorantes, A. Ivan Salido-Guadarrama, and Pilar Garcia-Tobilla
8. DNA Methylation Analysis of Steroid Hormone Receptor Genes
Ignacio Camacho-Arroyo, Valeria Hansberg-Pastor, and Mauricio Rodriguez-Dorantes
9. Control of Oncogenic miRNA Function by Light-Activated miRNA Antagomirs
Colleen M. Connelly and Alexander Deiters
10. Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling
Yi Feng, Xiaowen Hu, Youyou Zhang, Dongmei Zhang, Chunsheng Li, and Lin Zhang
Part III: Metastasis Promotion
11. Microvesicles as Mediators of Intercellular Communication in Cancer
Marc A. Antonyak and Richard A. Cerione
12. In Vivo Rat Model to Study Horizontal Tumor Progression
Catalina Trejo-Becerril, Enrique Perez-Cardenas, and Alfonso Duenas-Gonzalez
13. An In Vivo Model to Study the Effects of Tumoral Soluble Factors on the Vascular Permeability in Mice
Cesar Alejandro Guzman-Perez, Alfredo Ibarra-Sanchez, Jose Luis Ventura-Gallegos, Claudia Gonzalez-Espinosa, Jonathan Garcia-Roman, and Alejandro Zentella-Dehesa
14. Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis
Qi Lv, Fang Hua, and Zhuo-Wei Hu
15. Zebrafish-Based Systems Pharmacology of Cancer Metastasis
Yasuhito Shimada, Yuhei Nishimura, and Toshio Tanaka
Part IV: New Technologies in the Study of Cancer
16. A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells
Nohemi Salinas-Jazmin, Emiliano Hisaki-Itaya, and Marco A. Velasco-Velazquez
17. Gene Disruption Using Zinc Finger Nuclease Technology
Sara Granja, Ibtissam Marchiq, Fatima Baltazar, and Jacques Pouyssegur