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Cell Culture Techniques Michael Aschner

Cell Culture Techniques By Michael Aschner

Cell Culture Techniques by Michael Aschner


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Summary

Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use.

Cell Culture Techniques Summary

Cell Culture Techniques by Michael Aschner

Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use. Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing. Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing. As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory.

Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges.

Table of Contents

1. Guidance on Good Cell Culture Practice (GCCP)

Anna Bal-Price and Sandra Coecke

2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology

M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman

3. Neural Stem Cells

Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra Ceccatelli

4. Primary Cultures for Neurotoxicity Testing

Carme Sola, Rosa Cristofol, Cristina Sunol, and Coral Sanfeliu

5. Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening

Paul Honegger and Marie-Gabrielle Zurich

6. Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans

Michelle L. Tucci, Guy A. Caldwell, and Kim A. Caldwell

7. Modeling the Blood-Brain Barrier

Marie-Pierre Dehouck, Elodie Vandenhaute, Lucie Dehouck, Emmanuel Sevin, Anne-Marie Lenfant, Yannick Delplace, Dorothee Hallier-Vanuxeem, Maxime Culot, and Romeo Cecchelli

8. In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research

Nathalie Strazielle and Jean-Francois Ghersi-Egea

9. Introducing Cloned Genes into Cultured Neurons Providing Novel In vitro Models for Neuropathology and Neurotoxicity Studies

Marcelo Farina, Jordi Berenguer, Sebastian Pons, Joao Batista Teixeira da Rocha, and Michael Aschner

10. P19 Embryonic Carcinoma Cell Line: A Model To Study Gene-Environment Interactions

Joseph Bressler, Cliona O'Driscoll, Cathleen Marshall, and Walter Kaufmann

11. Signal Transduction and Neurotoxicity: What Can We Learn from Experimental Culture Systems?

Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti

12. Neurite Degeneration in Human Neuronal SH-SY5Y Cells as an Indicator of Axonopathy

Anna Forsby

13. The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity

Alan J. Hargreaves, Magda Sachana, and John Flaskos

14. Assessing Toxic Injuries of Experimental Therapeutics to the Crystalline Lens Using Lens Explant Culture

Michael D. Aleo

15. Necrosis, Apoptosis, and Autophagy: Mechanisms of Neuronal and Glial Cell Death

Michael Fricker and Aviva M. Tolkovsky

16. Inflammation and Reactive Oxygen/Nitrogen Species in Glial/Neuronal Cultures

Jonas J. Neher, Guy C. Brown, Agnieszka Kinsner-Ovaskainen, and Anna Bal-Price

17. Neuronal Oxidative Injury and Biomarkers of Lipid Peroxidation

Dejan Milatovic and Michael Aschner

18. Analysis of Protein Targets by Oxidative Stress Using the Oxyblot and Biotin-Avidin-Capture Methodology

Jeannette N. Stankowski, Simona G. Codreanu, Daniel C. Liebler, and BethAnn McLaughlin

19. Catecholaminergic Cell Lines for the Study of Dopamine Metabolism and Neurotoxicity

Juan Segura-Aguilar

20. 13C NMR Spectroscopy and Mass Spectrometry Analysis of Intermediary Metabolism in Cultured Neural Cells

Ursula Sonnewald, Arne Schousboe, and Helle S. Waagepetersen

21. Culture Models for the Study of Amino Acid Transport and Metabolism

Marta Sidoryk-Wegrzynowicz and Michael Aschner

22. Neurotransmitter Transporters and Anticonvulsant Drug Development

Arne Schousboe, Karsten K. Madsen, and H. Steve White

23. Ion Channel Electrophysiology in Cultured Neurons

Toshio Narahashi

24. Neurotoxicity Assessment by Recording Electrical Activity from Neuronal Networks on Microelectrode Array Neurochips

Dieter G. Weiss

25. GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing

Cristina Sunol and Daniel A. Garcia

Additional information

NLS9781493958139
9781493958139
1493958135
Cell Culture Techniques by Michael Aschner
New
Paperback
Humana Press Inc.
2016-08-23
497
N/A
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