PaPart I Tropisms
1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient
Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez
2. Assessing Gravitropic Responses in Arabidopsis
Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson
3. Physiological Analysis of Phototropic Responses in Arabidopsis
Mathias Zeidler
4. Automatic Chloroplast Movement Analysis
Henrik Johansson and Mathias Zeidler
5. Micro
scopic and Biochemical Visualization of Auxins in Plant Tissues
Joshua J. Blakeslee and Angus S. Murphy
6. Immunolocalization of PIN and ABCB Transporters in Plants
Nicola Carraro and Wendy A. Peer
Part II Photoperiodism and Circasian Rhythms
7.
Analysis of Circadian Leaf Movements
Niels A. Muller and Jose M. Jimemez-Go
mez
8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes
Fernando Andres, Stefano Torti, Coral Vincent, and George Coupland
9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are
Temporally Restricted by the Circadian Clock
Amaury de Montaigu, Markus C. Berns, and Geroge Coupland
10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome
Ghislain Breton, Steve A. Kay, and Jose L. Pruneda-Paz
11. Monitoring Alternative Splicing Changes in
Arabidopsis Circadian Clock Genes
Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger
12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian Clock
Michael J. Haydon and Alex A. R. Webb
13. Assessing Protein Stability Under Different Light and Circadian Conditions
Takatoshi Kiba and Rossana Henriques
Part III Abiotic Stress Responses
14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought
Diego M. Almeida, M. Cecilia Almadanim, Tiago Louren
c
o, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira
15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana
Urszula Piskurewicz and Luis Lopez-Molina
16. Assessing Tolerance to Heavy-Metal Stress in
Arabidopsis Thaliana Seedings
Estelle Remy and Paula Duque
17. Assessing Drought Responses Using Thermals Infrared Imaging
Ankush Prashar and Hamlyn G. Jones
18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms
Monika Maronova and Maria Kalyna
19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures
Imma P. Salamo, Bogata Boros, and L
aszlo Szabados
20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress
Ana Confraria and Elena Baena-Gonzalez
21. Construction of Artificial miRNAs to Prevent Drought Stress in
Solanum Tuberosum
Anna Wyrzykowska, Marcin Pieczynski, and Zofia Szweykowska-Kulinska
Part IV Plant-Pathogen Interactions 22. Virus-Induced Gene Silencing for Gene Function Studies in Barley
Maria Barciszewska-Pacak, Artur Jarmolowski, and Andrzej Pacak 23. Methods for Long-Term Stable Storage of
Colletotrichum Species
Kei Hiruma and Yusuke Saijo 24. Plant Inoculation with the Fungal Leaf Pathogen
Colletotrichum Higginsianum Kei Hiruma and Yusuke Saijo
25. Tracing Plant Defense Responses in Roots Upon MAMP/DAMP Treatment
Kei Hiruma and Yusuke Saijo
26. Analysis of the Immunity=Related Oxidative Bursts by a Luminol-Based Assay
Marco Trujillo
27. Quantitative Analysis of Microbe-Associated-Molecular-Pattern (MAMP)-Induced Ca2+ Transients in Plants
Fabian Trempel, Stefanie Ranf, Dierk Scheel, and Justin Lee
28. Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR
Stephanie Rausch and Sascha Laubinger
29. Determining Nucleosome Position at Individual Loci after Biotic Stress Using MNase-qPCR
Margaux Kaster and Sascha Laubinger
30. Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in
Arabidopsis Thaliana
Ines Lassowskat, Wolfgang Hoehenwarter, Justin Lee, and Dierk Scheel2.
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