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Lymphoma Ralf Kuppers

Lymphoma By Ralf Kuppers

Lymphoma by Ralf Kuppers


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Summary

Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers.

Lymphoma Summary

Lymphoma: Methods and Protocols by Ralf Kuppers

Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research. Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.

Table of Contents

1. Origin and Pathogenesis of B Cell Lymphomas

Marc Seifert, Rene Scholtysik, and Ralf Kuppers

2. Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma

David Wu, Brent L. Wood, and Jonathan R. Fromm

3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells

Ralf Kuppers, Markus Schneider, and Martin-Leo Hansmann

4. PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics

Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group

5. Expression Cloning of Human B Cell Immunoglobulins

Hedda Wardemann and Juliane Kofer

6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR

Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen

7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies

Martin J.S. Dyer

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

Nikos Darzentas and Kostas Stamatopoulos

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

Sebastian Boettcher, Matthias Ritgen, and Michael Kneba

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

Miriam Sonnet, Constance Bar, Michael Rehli, Dieter Weichenhan, and Christoph Plass

12. Gene Expression Profile Analysis of Lymphomas

Katia Basso and Ulf Klein

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Martin J.S. Dyer

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

Nikos Darzentas and Kostas Stamatopoulos

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

Sebastian Boettcher, Matthias Ritgen, and Michael Kneba

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

Miriam Sonnet, Constance Bar, Michael Rehli, Dieter Weichenhan, and Christoph Plass

12. Gene Expression Profile Analysis of Lymphomas

Katia Basso and Ulf Klein

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Katia Basso and Ulf Klein

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group

5. Expression Cloning of Human B Cell Immunoglobulins

Hedda Wardemann and Juliane Kofer

6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR

Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen

7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies

Martin J.S. Dyer

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

Nikos Darzentas and Kostas Stamatopoulos

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

Sebastian Boettcher, Matthias Ritgen, and Michael Kneba

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

Miriam Sonnet, Constance Bar, Michael Rehli, Dieter Weichenhan, and Christoph Plass

12. Gene Expression Profile Analysis of Lymphomas

Katia Basso and Ulf Klein

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Martin J.S. Dyer

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

Nikos Darzentas and Kostas Stamatopoulos

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

Sebastian Boettcher, Matthias Ritgen, and Michael Kneba

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

Miriam Sonnet, Constance Bar, Michael Rehli, Dieter Weichenhan, and Christoph Plass

12. Gene Expression Profile Analysis of Lymphomas

Katia Basso and Ulf Klein

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Katia Basso and Ulf Klein

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Additional information

NLS9781493959945
9781493959945
1493959948
Lymphoma: Methods and Protocols by Ralf Kuppers
New
Paperback
Humana Press Inc.
2016-08-23
315
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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