1. Assaying Radiosensitivity of Ataxia-Telangiectasia
Hailiang Hu, Shareef Nahas, and Richard A. Gatti
2. Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology
Amanda W. Kijas and Martin F. Lavin
3. ATM Gene Mutation Detection Techniques and Functional Analysis
Guillaume Rieunier, Catherine Dubois D'Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern
4. An HTRF (R) Assay for the Protein Kinase ATM
Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater
5. ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using CellomicsTM ArrayScan VTI Platform
Catherine Bardelle and Joanna Boros
6. Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci
Yi Chieh Lim
7. Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy
Keiji Suzuki
8. Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging
Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla
9. Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein
Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
10. Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry
Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov
11. Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS)
Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo
12. Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies
Sun Yingli and Du Fengxia
13. Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays
Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna
14. ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry
Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz
15. Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling
Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich
16. Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosphorylation
Marina E. Borisova, Sebastian A. Wagner, and Petra Beli
17. Statistical Analysis of ATM-Dependent Signaling in Quantitative Mass Spectrometry Phosphoproteomics
Ashley J. Waardenberg
18. ChIP Technique to Study Protein Dynamics at Defined DNA Double-Strand Breaks
Jie Wen and Patrick Concannon
19. Studies of the DNA Damage Response by Using the Lac Operator/Repressor (LacO/LacR) Tethering System
Rossana Piccinno, Marta Cipinska, and Vassilis Roukos
20. Imaging of Fluorescently-Tagged ATM Kinase at the Sites of DNA Double-Strand Breaks
Anthony J. Davis, Shih-Ya Wang, David J. Chen, and Benjamin P.C. Chen
21. Live Cell Imaging to Study Real-Time ATM-Mediated Recruitment of DNA Repair Complexes to Sites of Ionizing Radiation-Induced DNA Damage
Burkhard Jakob and Gisela Taucher Scholz
22. Analyzing Heterochromatic DNA Double Strand Break (DSB) Repair in Response to Ionizing Radiation
Karolin Klement and Aaron A. Goodarzi
23. Phenotypic Analysis of ATM Protein Kinase in DNA Double-Strand Break Formation and Repair Elisabeth Mian and Lisa Wiesmuller
24. Monitoring DNA Repair Consequences of ATM Signaling Using Simultaneous Fluorescent Readouts
Adrian Wiegmans
25. Non-Canonical ATM Activation and Signaling in Response to Transcription-Blocking DNA Damage
Jurgen A. Marteijn, Wim Vermeulen, and Maria Tresini
26. Study of ATM Phosphorylation by Cdk5 in Neuronal Cells Hua She and Zixu Mao
27. DNA Damage Response in Human Stem Cells and Neural Descendants
Jason M. Beckta, Bret R. Adams, and Kristoffer Valerie
28. A Patient-Specific Stem Cell Model to Investigate the Neurological Phenotype Observed in Ataxia-Telangiectasia
Romal Stewart, Gautam Wali, Chris Perry, Martin Lavin, Francois Feron, Alan Mackay-Sim, and Ratneswary Sutharsan
29. Lentiviral Reprogramming of A-T Patient Fibroblasts to Induced Pluripotent Stem Cells
Sam Nayler, Sergei Kozlov, Martin Lavin, and Ernst Wolvetang
30. Monitoring the ATM-Mediated DNA Damage Response in the Cerebellum Using Organotypic Cultures
Efrat Tal and Yosef Shiloh