Microdissection of Histological Sections: Manual and Laser Capture Microdissection Techniques Christopher A. Moskaluk Isolation of a Purified Epithelial Cell Population from Human Colon James K. Roche Xenografting Human Colon Cancers Jeffrey C. Harper, Reid B. Adams, and Steven M. Powell Comparative Genomic Hybridization Technique Wa'el El-Rifai and Sakari Knuutila Fluorescence In Situ Hybridization: Application in Cancer Research and Clinical Diagnostics Svetlana D. Pack and Zhengping Zhuang Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer Jin Jen The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity Darryl Shibata Microsatellite Instability Testing Yann R. Parc and Kevin C. Halling Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer Kevin C. Halling and Patrick C. Roche Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes Julie M. Cunningham, David J. Tester, and Stephen N. Thibodeau Mutation Detection in the TGF-b Receptors William M. Grady, Lois L. Myeroff, Hongmei He, and Sanford Markowitz Direct Analysis for Familial Adenomatous Polyposis Mutations Steven M. Powell I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer Perry S. Chan and Steven L. Gersen Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas Rhonda F. Souza and Stephen J. Meltzer Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors Sam Thiagalingam Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations Lauri A. Aaltonen and Stina Roth Direct Sequencing for Peutz-Jehgers Gene LKB1 (STK11) Mutations Lauri A. Aaltonen and Egle Avizienyte Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations Lauri A. Aaltonen, Stina Roth, and Charis Eng Genetic Analysis Using Microarrays Wa'el El-Rifai and Sakari Knuutila Cyclooxygenase-2 (COX-2) Protein Expression by Western Blotting Hossam M. Kandil and Raymond N. DuBois The Immunohistochemical Method Lisa A. Cerilli and Henry F. Frierson, Jr Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids Rosana Cosme and James K. Roche Adhesion Signaling Through Integrins Reid B. Adams and Joshua D. Rovin Electrophoretic Mobility Shift Assay (EMSA) Michael F. Smith, Jr. and Sandrine Delbary-Gossart Transfection Assays for Transformation of Model Colonic Cell Lines Mark T. Worthington and Roger Qi Luo Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay Kimberly S. Tustison, Joanne Yu, and Steven M. Cohn Gene Transfer into the Colonic Mucosa Bacak Coruh and Theresa T. Pizarro Index
