Section I: Recombinant Expression of Membrane Proteins 1.Engineering Escherichia Coli for Functional Expression of Membrane Proteins Franz Y Ho and Bert Poolman 2.ACEMBLing a Multiprotein Transmembrane Complex: The Functional SecYEG-SecDFYajC-YidC Holotranslocon Protein Secretase/Insertase Joanna Komar, Mathieu Botte, Ian Collinson, Christiane Schaffitzel and Imre Berger 3. Expression and Purification of OsVDAC4 Ashwini Godbole, Ashvini K. Dubey, Rohan Mitra, Palakolanu S. Reddy and M. K. Mathew 4.Membrane Protein Expression in Lactococcus Lactis Martin S. King, Christoph Boes and Edmund R.S. Kunji 5.An Unconventional Anaerobic Membrane Protein Production System Based on Wolinella Succinogenes Michael Lafontaine and C. Roy D. Lancaster 6.Membrane Protein Expression and Analysis in Yeast Katrien Claes, Mouna Guerfal and Nico Callewaert 7.Heterologous Expression of G Protein Coupled Receptors in Yeast Nicolas Bertheleme, Shweta Singh, Simon Dowell and Bernadette Byrne 8.Recombinant G protein-Coupled Receptor Expression in Saccharomyces Cerevisiae for Protein Characterization Kory M. Blocker, Zachary T. Britton, Andrea N. Naranjo, Patrick M. McNeely, Carissa L. Young and Anne S. Robinson 9.Baculovirus Mediated Expression of GPCRs in Insect Cells Tuulia Saarenpaa, Veli-Pekka Jaakola and Adrian Goldman 10.Expression of Membrane Proteins in the Eyes of Transgenic Drosophila Melanogaster Yvonne Hackmann, Lisa Joedicke, Valerie Panneels and Irmgard Sinning 11.Rapid Method to Express and Purify Human Membrane Protein Using the Xenopus Oocyte System for Functional and Low-Resolution Structural Analysis Benjamin Clemencon, Michael Fine, Philipp Schneider and Matthias A. Hediger 12.Expression of G protein-coupled Receptors in Mammalian Cells Raja Chakraborty, Bing Xu, Rajinder P. Bhullar and Prashen Chelikani 13.Construction of Stable Mammalian Cell Lines for Inducible Expression of G Protein-Coupled Receptors Chikwado A. Opefi, Dale Tranter, Steven O. Smith and Philip J. Reeves. 14.Rapid and Facile Recombinant Expression of Bovine Rhodopsin in HEK293S GnTI Cells Using a PiggyBac Inducible System Lydia N. Caro, Zhijie Li, James M. Rini, Aidin R. Balo and Oliver P. Ernst 15.Semliki Forest Virus-based Expression of Recombinant GPCRs Kenneth Lundstrom 16.Screening for Lipid Requirements of Membrane Proteins by Combining Cell-Free Expression with Nanodiscs Erik Henrich, Volker Dotsch, and Frank Bernhard Section II. Reconstitution and Functional Characterization of Membrane Proteins 17.Liposome Reconstitution and Transport Assay for Recombinant Transporters Zachary Lee Johnson and Seok-Yong Lee 18.Lipid Reconstitution and Recording of Recombinant Ion Channels Randy Stockbridge and Ming-Feng Tsai 19.Reconstitution of Membrane Proteins - a GPCR as an Example Alan D. Goddard, Patricia M. Dijkman, Roslin J. Adamson, Rosana Inacio dos Reis and Anthony Watts 20.Ion Channel Reporter for Monitoring the Activity of Engineered GPCRs Christophe J. Moreau, Katarzyna Niescierowicz , Lydia Caro , Jean Revilloud and Michel Vivaudou 21.Methods for Labeling Skeletal Muscle Ion Channels Site-Specifically With Fluorophores Suitable for FRET-based Structural Analysis Mohana Mahalingam and James D. Fessenden 22.Structural and Functional Studies of Nirc From Salmonella Typhimurium Adriana Rycovska-Blume,Wei Lu, Susana Andrade, Klaus Fendler and Oliver Einsle 23.Surface Plasmon Resonance Analysis of 7-Transmembrane Receptors Tonia Aristotelous, Andrew L. Hopkins and Iva Navratilova 24.Crosslinking Strategies to Study Peptide Ligand-Receptor Interactions Jeffery Becker 25.From recombinant expression to crystals A step-by-step guide to GPCR crystallography Arun K. Shukla, Punita Kumari, Eshan Ghosh and Kumari Nidhi 26.Structure-based Biophysical Analysis of the Interaction of Rhodopsin with G Protein and Arrestin Martha E. Sommer, Matthias Elgeti, Peter W. Hildebrand, Michal Szczepek, Klaus Peter Hofmann and Patrick Scheerer
