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Ion Channels: Channel Production and Optical Methods Volume editor Daniel L. Minor (Professor, University of California San Francisco, CA, USA)

Ion Channels: Channel Production and Optical Methods By Volume editor Daniel L. Minor (Professor, University of California San Francisco, CA, USA)

Ion Channels: Channel Production and Optical Methods by Volume editor Daniel L. Minor (Professor, University of California San Francisco, CA, USA)


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Ion Channels: Channel Production and Optical Methods Summary

Ion Channels: Channel Production and Optical Methods: Volume 653 by Volume editor Daniel L. Minor (Professor, University of California San Francisco, CA, USA)

Ion Channels Part B, Volume 652 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including NMDAR, Pannexin, and CALHM, Making NaV1.4 and NaV1.7, TRPVs, Purification native nAChRs, GABAR Radu Aricescu, TRPV5/2, NaV1.5, KATP, TRPA1, TREK-1, SARS-CoV-2 3a ion channel, Ion channel conformational dynamics by encoded unnatural amino acid, Fluorescence lifetime measurement of absolute membrane potential, Fluorescent Toxins as Activity Sensors, FRET Analyses of Ion Channel Protein-Protein Interactions, Control of Ion Channel Gating with Photo-Switchable Tweezers, and Counting Subunits in Kv Channel Complexes.

About Volume editor Daniel L. Minor (Professor, University of California San Francisco, CA, USA)

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Table of Contents

Preface Daniel L. Minor Jr. and Henry M. Colecraft Methods to produce and purify ion channels 1. Effective production of oligomeric membrane proteins by EarlyBac Hiro Furukawa 2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies Lejla Zubcevic 3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies Vera Moiseekova-Bell 4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies Seok-Yong Lee 5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination William A. Catterall 6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy Nieng Yan 7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy Show-Ling Shyng 8. Production of K2P2.1 (TREK-1) for structural studies Daniel L. Minor Jr. 9. Purification of a native nicotinic receptor Ryan Hibbs 10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs Stephen Brohawn Optical methods for probing and controlling channel function 11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid Sebastian E. Brauchi 12. Fluorescence lifetime measurement of absolute membrane potential Evan Miller 13. Fluorescent Toxins as Ion Channel Activity Sensors Jon Sack 14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer Manu Ben-Johny 15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers Thomas Grutter 16. Counting Subunits in Kv Channel Complexes Rikard Blunck

Additional information

NPB9780323853767
9780323853767
0323853765
Ion Channels: Channel Production and Optical Methods: Volume 653 by Volume editor Daniel L. Minor (Professor, University of California San Francisco, CA, USA)
New
Hardback
Elsevier Science & Technology
2021-06-09
420
N/A
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