1.Methods for Monitoring Lysosomal Morphology Bethan S. Kilpatrick, Emily R. Eden, Leanne N. Hockey, Clare E. Futter and Sandip Patel 2.A Rapid Method for the Preparation of Ultra-pure, Functional Lysosomes Using Funcionalised Superparamagnetic Iron Oxide Nanoparticles Mathew William Walker and Emyr Lloyd-Evans 3.TFEB and the CLEAR Network Carmine Settembre and Diego L. Medina 4.Biosynthesis, Targeting and Processing of Lysosomal Proteins: Pulse-Chase Labeling and Immune Precipitation Sandra Pohl and Andrej Hasilik 5.Measuring Lysosomal pH by Fluorescence Microscopy Johnathan Canton and Sergio Grinstein 6.Lysosome Fusion in Cultured Mammalian Cells Nicholas A. Bright, Lena Wartosch and J. Paul Luzio 7.RNAi Screens of Lysosomal Trafficking Salil Garg, Michael B. Brenner 8.Approaches for Plasma Membrane Wounding and Assessment of Lysosome-mediated Repair Responses Corrotte, M, Castro-Gomes, T, Koushik, A.B. and Andrews, N.W 9.Imaging Approaches to Measuring Lysosomal Calcium Anthony J. Morgan, Lianne C. Davis, Antony Galione 10.Lysosome Electrophysiology Xi Zoe Zhong and Xian-Ping Dong 11.Reconstitution of Lysosomal Ion Channels into Artificial Membranes Elisa Venturi and Rebecca Sitsapesan 12.Fluorescence Methods for Analysis of Interactions Between Ca2+ Signalling, Lysosomes and Endoplasmic Reticulum David L. Prole, Cristina I. Lopez-Sanjurjo, Stephen C. Tovey and Colin W. Taylor 13.Methods for the Quantification of Lysosomal Membrane Permeabilization: a Hallmark of Lysosomal Cell Death Sonja Aits, Marja Jaattela and Jesper Nylandsted 14.Measuring the Phagocytic Activity of Cells Nick Platt and Paul Fineran 15.Detection and Quantification of Microbial Manipulation of Phagosomal Function Maria Podinovskaia, David G. Russell 16.Measuring Relative Lysosomal Volume for Monitoring Lysosomal Storage Diseases Danielle te Vruchte, Kerri L. Wallom and Frances M. Platt 17.Quantifying Storage Material Accumulation in Tissue Sections Jonathan D Cooper, Helen R Brooks, Hemanth R Nelvagal 18.Laboratory Diagnosis of Niemann-Pick disease Type C: The Filipin Staining Test Marie T. Vanier and Philippe Latour
