Name: Laboratory Methods in Enzymology: Protein Part A By Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)
SKU: 9780124200708
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Laboratory Methods in Enzymology: Protein Part A Summary

Laboratory Methods in Enzymology: Protein Part A: Volume 536 by Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: Pulse-chase analysis to measure protein degradation Labeling a protein with fluorophores using NHS ester derivitization Immunoaffinity purification of proteins Proteolytic affinity tag cleavage Purification of GST-tagged proteins

Practical Steady-state Enzyme Kinetics Quantification of Protein Concentration using UV absorbance and Coomassie Dyes Preparation of protein samples for mass spectrometry and N-terminal sequencing Lysis of mammalian and Sf9 cells In Vivo [35 S]-Methionine Incorporation Pulse-chase analysis to measure protein degradation Labeling of a Protein with Fluorophores using Maleimide Derivitization Labeling a protein with fluorophores using NHS ester derivitization Protein Derivitization-Expressed Protein Ligation Protein Biotinylation Purification of His-tagged proteins Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag Immunoaffinity purification of proteins Affinity Purification of Protein Complexes Using TAP Tags Strep-tagged protein purification Proteolytic affinity tag cleavage Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads Protein Affinity Purification using Intein/Chitin Binding Protein Tags Purification of GST-tagged proteins

Additional information

Sku

NPB9780124200708

ISBN 13

9780124200708

ISBN 10

0124200702

Title

Laboratory Methods in Enzymology: Protein Part A: Volume 536 by Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Author

Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Condition

New

Binding type

Hardback

Publisher

Elsevier Science Publishing Co Inc

Year published

2014-03-25

Number of pages

200

Prizes

N/A

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Book picture is for illustrative purposes only, actual binding, cover or edition may vary.

Note

This is a new book - be the first to read this copy. With untouched pages and a perfect binding, your brand new copy is ready to be opened for the first time

Laboratory Methods in Enzymology: Protein Part A Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Laboratory Methods in Enzymology: Protein Part A by Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Summary

Laboratory Methods in Enzymology: Protein Part A Summary

Laboratory Methods in Enzymology: Protein Part A: Volume 536 by Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Table of Contents

Additional information

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