Name: Laboratory Methods in Enzymology: Protein Part B By Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)
SKU: 9780124201200
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Laboratory Methods in Enzymology: Protein Part B Summary

Laboratory Methods in Enzymology: Protein Part B: Volume 539 by Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.

Small-scale expression of proteins in E. coli Protein expression-Yeast Recombinant protein expression in baculovirus-infected insect cells Single cell cloning of a stable mammalian cell line In vitro synthesis of proteins in bacterial extracts Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation Yeast two-hybrid screen UV cross-linking of interacting RNA and protein in cultured cells Analysis of RNAprotein interactions by cell mixing General proteinprotein cross-linking Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation):A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system Dissecting a known RNAprotein interaction using a yeast three-hybrid system Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system

Additional information

Sku

NPB9780124201200

ISBN 13

9780124201200

ISBN 10

0124201202

Title

Laboratory Methods in Enzymology: Protein Part B: Volume 539 by Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Author

Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Condition

New

Binding type

Hardback

Publisher

Elsevier Science Publishing Co Inc

Year published

2014-04-29

Number of pages

240

Prizes

N/A

Cover note

Book picture is for illustrative purposes only, actual binding, cover or edition may vary.

Note

This is a new book - be the first to read this copy. With untouched pages and a perfect binding, your brand new copy is ready to be opened for the first time

Laboratory Methods in Enzymology: Protein Part B Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Laboratory Methods in Enzymology: Protein Part B by Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Laboratory Methods in Enzymology: Protein Part B Summary

Laboratory Methods in Enzymology: Protein Part B: Volume 539 by Volume editor Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)

Table of Contents

Additional information

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