1. Dynamic Imaging of the Recycling Endosomal Network in Macrophages Adam A. Wall, Nicholas D. Condon, Jeremy C. Yeo, Nicholas A. Hamilton and Jennifer L. Stow 2. Analyzing the Functions of Rab11-Effector Proteins During Cell Division Rytis Prekeris 3. Expression and Localization of Exocytic and Recycling Rabs from Magnaportheoryzae in Mammalian Cells Yaoyao Qi, M. Caleb Marlin, Zhimin Liang, Dongmei Zhang, Jie Zhou, Zonghua Wang, Guodong Lu and Guangpu Li 4. Methods to Analyze Novel Liaisons Between Endosomes and Centrosomes Hui-Fang Hung, Heidi Hehnly and Stephen Doxsey 5. Biochemical Analysis of Rabin8, the Guanine Nucleotide Exchange Factor for Rab8 Bin Wu, Juanfei Wang, Yuting Zhao and Wei Guo 6. Simple In vitro Assay of Arf GAPs and Preparation of Arf Proteins as Substrates Pei-Wen Chen, Xiaoying Jian, Ruibai Luo and Paul A. Randazzo 7. An ACAP1 Coat Complex Acting in Endocytic Recycling Jian Li and Victor W. Hsu 8. Biochemical Methods for Studying Kinetic Regulation of Arf1 Activation by Sec7 Brian C. Richardson and J. Christopher Fromme 9. Rab and Arf G Proteins in Endosomal Trafficking Dipannita Dutta and Julie G. Donaldson 10. Qualitative and Quantitative Analysis of Endocytic Recycling James B. Reineke, Shuwei Xie, Naava Naslavsky and Steve Caplan 11. Methods for Analyzing the Role of Phospholipase A2 Enzymes in Endosome Membrane Tubule Formation Danielle N. Kalkofen, Paul de Figueiredo and William J. Brown 12. In vivo Analysis of Recycling Endosomes in C. elegans Anbing Shi and Barth D. Grant 13. Monitoring Receptor Trafficking Following Retromer and WASH Deregulation Douglas G. Osborne, Christine A Phillips-Krawczak and Daniel D. Billadeau 14. Quantitative Analysis of Endosome Tubulation and Microdomain OrganizationMediated by the WASH Complex Emmanuel Derivery and Alexis Gautreau 15. Rab GTPases and Kinesin Motors in Endosomal Trafficking Cedric Delevoye and Bruno Goud 16. Measuring Receptor Recycling in Polarized MDCK Cells Luciana Gallo and Gerard Apodaca 17. Structural and Functional Analysis of Endosomal Compartments in Epithelial Cells Andres E. Perez Bay, Ryan Schreiner and Enrique Rodriguez-Boulan 18. Analyzing the Role of AP-1B in Polarized Sorting from Recycling Endosomes in Epithelial Cells Heike Folsch 19. Recycling of the Insulin Responsive Glucose Transporter Glut4 Regulated by the Small GTPase RalA and the Exocyst Complex Chao Nie and Xiao-Wei Chen 20. GPCR Sorting at the Multivesicular Endosomes Michael Robert Dores and JoAnn Trejo 21. Visualizing and Quantitating Sequence-Dependent GPCR Recycling Shanna L. Bowman, Amanda L. Soohoo and Manojkumar A. Puthenveedu 22. Methods to Study Endocytic Trafficking of the EGF Receptor Itziar Pinilla-Macua and Alexander Sorkin