Experimental Models of Parkinsons Disease by Yuzuru Imai
Authoritative and practical, Experimental Models of Parkinson's Disease is an ideal guide for researchers continuing to work toward making PD risk predictable and surmountable.
-Synuclein Seeding Assay UsingRT-QuIC.-Electron-Microscopic Analysis of -Synuclein Fibrils.- -Synuclein Seeding Assay Using Cultured Cells.- Analysis of -Synuclein in Exosomes.- Measurement of GCase Activity in Cultured Cells.- Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells.- Two Methods to Analyze LRRK2 Functions Under Lysosomal Stress: The Measurements of Cathepsin Release and Lysosomal Enlargement.- Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells.- Monitoring PINK1-Parkin Signaling Using Dopaminergic Neurons from iPS Cells.- Generation of Mitochondrial Toxin Rodent Models of Parkinsons Disease Using 6-OHDA, MPTP, and Rotenone.- Midbrain Slice Culture as an Ex Vivo Analysis Platform for Parkinson's Disease.- -Synuclein Propagation Mouse Models of Parkinsons Disease.- Common Marmoset Model of -Synuclein Propagation.- Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections.-Deep Brain Stimulation Using Animal Models of Parkinsons Disease.-Assessment of Cytotoxicity of -Synuclein in Budding Yeast Using a Spot Growth Assay and Fluorescent Microscopy.-The Functional Assessment of LRRK2 in Caenorhabditis elegans Mechanosensory Neurons.- Analysis of Dopaminergic Functions inDrosophila.-Evaluation of Mitochondrial Function and Morphology in Drosophila.-Cytosolic and Mitochondrial Ca2+ Imaging in Drosophila Dopaminergic Neurons.